Separation and identification of volatile components in the fermentation broth of Trichoderma atroviride by solid-phase extraction and gas chromatography-mass spectrometry

Ágnes Keszler, E. Forgács, László Kótai, Juan Antonio Vizcaíno, Enrique Monte, Isabel García-Acha

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A preseparated fermentation broth of Trichoderma atroviride strain 11 is analyzed by gas chromatography followed by mass-spectral detection using a Finnigan MAT GCQ apparatus. After preseparation in a C18 and a silica gel column, nineteen pyrone and dioxolane derivatives and two aliphatic esters are obtained, respectively. Among these, the four dioxolane derivatives have not been identified previously. The main component is found to be 5,5′-dimethyl-2H-pyran-2-on. The relative standard deviation for the determination of the retention time and the peak area (measured in ion counts) is 0.1% and 4.5%, respectively.

Original languageEnglish
Pages (from-to)421-424
Number of pages4
JournalJournal of Chromatographic Science
Volume38
Issue number10
Publication statusPublished - Oct 2000

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Trichoderma
Solid Phase Extraction
Gas chromatography
Gas Chromatography-Mass Spectrometry
Fermentation
Mass spectrometry
Pyrones
Derivatives
Pyrans
Silica Gel
Gas Chromatography
Esters
Ions
formal glycol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Separation and identification of volatile components in the fermentation broth of Trichoderma atroviride by solid-phase extraction and gas chromatography-mass spectrometry. / Keszler, Ágnes; Forgács, E.; Kótai, László; Vizcaíno, Juan Antonio; Monte, Enrique; García-Acha, Isabel.

In: Journal of Chromatographic Science, Vol. 38, No. 10, 10.2000, p. 421-424.

Research output: Contribution to journalArticle

Keszler, Ágnes ; Forgács, E. ; Kótai, László ; Vizcaíno, Juan Antonio ; Monte, Enrique ; García-Acha, Isabel. / Separation and identification of volatile components in the fermentation broth of Trichoderma atroviride by solid-phase extraction and gas chromatography-mass spectrometry. In: Journal of Chromatographic Science. 2000 ; Vol. 38, No. 10. pp. 421-424.
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