Separation and identification of antibacterial chamomile components using OPLC, bioautography and GC-MS

Ágnes M. Móricz, Szabolcs Szarka, Péter G. Ott, Éva B. Héthelyi, Éva Szoke, Erno Tyihák

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Components of 50% aqueous ethanol chamomile (Matricaria recutica L.) flower extract, previously found antibacterial in a TLC-bioautographic study, were separated and isolated by the use of on-line overpressured layer chromatography (OPLC). This system consisted of an OPLC 50 BS system, an on-line coupled flow-through UV detector, and a manual fraction collector. The collected fractions were investigated by GC-MS analysis and by TLC re-chromatography with subsequent visualization, performed after use of the vanillin-sulphuric acid reagent, or under UV illumination, or applying bioautographic detection. The main compounds of the collected 11 fractions were identified by GC-MS. The results showed that the antibacterial effect of 50% aqueous ethanol extract of chamomile is ascribable to cis-, trans-spiroethers, and the coumarins like herniarin and umbelliferone.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalMedicinal Chemistry
Issue number1
Publication statusPublished - Jan 1 2012



  • Antibacterial activity
  • Bioautography
  • Chamomile
  • Coumarins
  • GC-MS
  • Matricaria recutita L.
  • OPLC
  • Spiroethers

ASJC Scopus subject areas

  • Drug Discovery

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