Separation and detection of paprika pigments by HPLC

B. Czinkotai, H. G. Daood, P. A. Biacs, F. Hajdu

Research output: Contribution to journalConference article

21 Citations (Scopus)

Abstract

Chromatographic separation of paprika pigments (Capsicum annuum) always faces the problem that oxidation and isomerization may take place during the separation process. Chromatographic separation of individual colour substances from paprika products has been carried out by using mobile phases containing either acetone or chloroform alone or as a gradient system. Various mobile phases were used for simultaneous fractionation of different pigments of paprika on Chromsil C18 column under isocratic conditions. Aceto-nitrile-isopropanol-water (39:57:4) seemed to be the best mobile phase for separation. Detection of individual pigments was carried out at different wavelength by means of a variable wavelength detector of Beckman. Compound identification was achieved by direct scanning of the maximum absorption spectra of each component during HPLC separation as well as by TLC separation. It was found that capsanthin and capsorubin react with fatty acids and form mono and diesters. Quantitative and qualitative changes occuring in the pigments of ground paprika during storage were also studied.

Original languageEnglish
Pages (from-to)2707-2717
Number of pages11
JournalJournal of Liquid Chromatography
Volume12
Issue number14
Publication statusPublished - Oct 1 1989
EventSelected Papers from the Symposium on Advances in Liquid Chromatography - Szeged, Hung
Duration: Sep 3 1988Sep 7 1988

ASJC Scopus subject areas

  • Molecular Medicine

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