Separation and detection of bioactive essential oil components by overpressured layer chromatography coupled with bioactivity tests

Ágnes M. Móricz, Dániel Krüzselyi, Péter G. Ott

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Overpressured layer chromatography (OPLC), ensuring pumpforced constant mobile phase flow and the possibility of overrun, offers the expanded exploitation of fne-particle adsorbent layers for a longer development distance. Using an infusion-transfusion OPLC method with a 26-cm long development, the separation of clove, rosemary, eucalyptus, tea tree, spearmint, thyme, and cinnamon bark essential oil components was achieved with good resolutions. In the combination of OPLC and Aliivibrio fscheri assay, the main essential oil components eugenol, borneol, (-)-R-carvone, thymol, and trans-cinnamaldehyde exhibited antibacterial e?ect. The OPLC-2,2-diphenyl-1-picrylhydrazyl (DPPH∗) test showed two antioxidant components: eugenol and thymol.

Original languageEnglish
Pages (from-to)121-125
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume30
Issue number2
DOIs
Publication statusPublished - Apr 2017

Keywords

  • Antioxidant 2,2-diphenyl-1-picrylhydrazyl test
  • Direct bioautography
  • Essential oils
  • High-performance thin-layer chromatography
  • Luminescent Aliivibrio fscheri
  • Overpressured layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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