Sensory neuropathy affects cardiac mirna expression network targeting igf-1, slc2a-12, eif-4e, and ulk-2 mrnas

Péter Bencsik, Krisztina Kiss, Bence Ágg, Júlia A. Baán, Gergely Ágoston, A. Varga, Kamilla Gömöri, Luca Mendler, Nóra Faragó, A. Zvara, P. Sántha, L. Puskás, G. Jancsó, Péter Ferdinandy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Here we examined myocardial microRNA (miRNA) expression profile in a sensory neuropathy model with cardiac diastolic dysfunction and aimed to identify key mRNA molecular targets of the differentially expressed miRNAs that may contribute to cardiac dysfunction. Methods: MaleWistar rats were treated with vehicle or capsaicin for 3 days to induce systemic sensory neuropathy. Seven days later, diastolic dysfunction was detected by echocardiography, and miRNAs were isolated from the whole ventricles. Results: Out of 711 known miRNAs measured by miRNA microarray, the expression of 257 miRNAs was detected in the heart. As compared to vehicle-treated hearts, miR-344b, miR-466b, miR-98, let-7a, miR-1, miR-206, and miR-34b were downregulated, while miR-181a was upregulated as validated also by quantitative real time polymerase chain reaction (qRT-PCR). By an in silico network analysis, we identified common mRNA targets (insulin-like growth factor 1 (IGF-1), solute carrier family 2 facilitated glucose transporter member 12 (SLC2a-12), eukaryotic translation initiation factor 4e (EIF-4e), and Unc-51 like autophagy activating kinase 2 (ULK-2)) targeted by at least three altered miRNAs. Predicted upregulation of these mRNA targets were validated by qRT-PCR. Conclusion: This is the first demonstration that sensory neuropathy affects cardiac miRNA expression network targeting IGF-1, SLC2a-12, EIF-4e, and ULK-2, which may contribute to cardiac diastolic dysfunction. These results further support the need for unbiased omics approach followed by in silico prediction and validation of molecular targets to reveal novel pathomechanisms.

Original languageEnglish
Article number991
JournalInternational journal of molecular sciences
Volume20
Issue number4
DOIs
Publication statusPublished - Feb 2 2019

Fingerprint

MicroRNAs
Polymerase chain reaction
Insulin
polymerase chain reaction
insulin
Echocardiography
vehicles
Eukaryotic Initiation Factor-4E
Microarrays
Electric network analysis
Eukaryotic Initiation Factors
echocardiography
network analysis
transporter
Glucose
Rats
Somatomedins
Demonstrations
glucose
Computer Simulation

Keywords

  • Capsaicin
  • Heart
  • Microrna
  • Network analysis
  • Sensory neuropathy

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Sensory neuropathy affects cardiac mirna expression network targeting igf-1, slc2a-12, eif-4e, and ulk-2 mrnas. / Bencsik, Péter; Kiss, Krisztina; Ágg, Bence; Baán, Júlia A.; Ágoston, Gergely; Varga, A.; Gömöri, Kamilla; Mendler, Luca; Faragó, Nóra; Zvara, A.; Sántha, P.; Puskás, L.; Jancsó, G.; Ferdinandy, Péter.

In: International journal of molecular sciences, Vol. 20, No. 4, 991, 02.02.2019.

Research output: Contribution to journalArticle

Bencsik, Péter ; Kiss, Krisztina ; Ágg, Bence ; Baán, Júlia A. ; Ágoston, Gergely ; Varga, A. ; Gömöri, Kamilla ; Mendler, Luca ; Faragó, Nóra ; Zvara, A. ; Sántha, P. ; Puskás, L. ; Jancsó, G. ; Ferdinandy, Péter. / Sensory neuropathy affects cardiac mirna expression network targeting igf-1, slc2a-12, eif-4e, and ulk-2 mrnas. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 4.
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abstract = "Background: Here we examined myocardial microRNA (miRNA) expression profile in a sensory neuropathy model with cardiac diastolic dysfunction and aimed to identify key mRNA molecular targets of the differentially expressed miRNAs that may contribute to cardiac dysfunction. Methods: MaleWistar rats were treated with vehicle or capsaicin for 3 days to induce systemic sensory neuropathy. Seven days later, diastolic dysfunction was detected by echocardiography, and miRNAs were isolated from the whole ventricles. Results: Out of 711 known miRNAs measured by miRNA microarray, the expression of 257 miRNAs was detected in the heart. As compared to vehicle-treated hearts, miR-344b, miR-466b, miR-98, let-7a, miR-1, miR-206, and miR-34b were downregulated, while miR-181a was upregulated as validated also by quantitative real time polymerase chain reaction (qRT-PCR). By an in silico network analysis, we identified common mRNA targets (insulin-like growth factor 1 (IGF-1), solute carrier family 2 facilitated glucose transporter member 12 (SLC2a-12), eukaryotic translation initiation factor 4e (EIF-4e), and Unc-51 like autophagy activating kinase 2 (ULK-2)) targeted by at least three altered miRNAs. Predicted upregulation of these mRNA targets were validated by qRT-PCR. Conclusion: This is the first demonstration that sensory neuropathy affects cardiac miRNA expression network targeting IGF-1, SLC2a-12, EIF-4e, and ULK-2, which may contribute to cardiac diastolic dysfunction. These results further support the need for unbiased omics approach followed by in silico prediction and validation of molecular targets to reveal novel pathomechanisms.",
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T1 - Sensory neuropathy affects cardiac mirna expression network targeting igf-1, slc2a-12, eif-4e, and ulk-2 mrnas

AU - Bencsik, Péter

AU - Kiss, Krisztina

AU - Ágg, Bence

AU - Baán, Júlia A.

AU - Ágoston, Gergely

AU - Varga, A.

AU - Gömöri, Kamilla

AU - Mendler, Luca

AU - Faragó, Nóra

AU - Zvara, A.

AU - Sántha, P.

AU - Puskás, L.

AU - Jancsó, G.

AU - Ferdinandy, Péter

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N2 - Background: Here we examined myocardial microRNA (miRNA) expression profile in a sensory neuropathy model with cardiac diastolic dysfunction and aimed to identify key mRNA molecular targets of the differentially expressed miRNAs that may contribute to cardiac dysfunction. Methods: MaleWistar rats were treated with vehicle or capsaicin for 3 days to induce systemic sensory neuropathy. Seven days later, diastolic dysfunction was detected by echocardiography, and miRNAs were isolated from the whole ventricles. Results: Out of 711 known miRNAs measured by miRNA microarray, the expression of 257 miRNAs was detected in the heart. As compared to vehicle-treated hearts, miR-344b, miR-466b, miR-98, let-7a, miR-1, miR-206, and miR-34b were downregulated, while miR-181a was upregulated as validated also by quantitative real time polymerase chain reaction (qRT-PCR). By an in silico network analysis, we identified common mRNA targets (insulin-like growth factor 1 (IGF-1), solute carrier family 2 facilitated glucose transporter member 12 (SLC2a-12), eukaryotic translation initiation factor 4e (EIF-4e), and Unc-51 like autophagy activating kinase 2 (ULK-2)) targeted by at least three altered miRNAs. Predicted upregulation of these mRNA targets were validated by qRT-PCR. Conclusion: This is the first demonstration that sensory neuropathy affects cardiac miRNA expression network targeting IGF-1, SLC2a-12, EIF-4e, and ULK-2, which may contribute to cardiac diastolic dysfunction. These results further support the need for unbiased omics approach followed by in silico prediction and validation of molecular targets to reveal novel pathomechanisms.

AB - Background: Here we examined myocardial microRNA (miRNA) expression profile in a sensory neuropathy model with cardiac diastolic dysfunction and aimed to identify key mRNA molecular targets of the differentially expressed miRNAs that may contribute to cardiac dysfunction. Methods: MaleWistar rats were treated with vehicle or capsaicin for 3 days to induce systemic sensory neuropathy. Seven days later, diastolic dysfunction was detected by echocardiography, and miRNAs were isolated from the whole ventricles. Results: Out of 711 known miRNAs measured by miRNA microarray, the expression of 257 miRNAs was detected in the heart. As compared to vehicle-treated hearts, miR-344b, miR-466b, miR-98, let-7a, miR-1, miR-206, and miR-34b were downregulated, while miR-181a was upregulated as validated also by quantitative real time polymerase chain reaction (qRT-PCR). By an in silico network analysis, we identified common mRNA targets (insulin-like growth factor 1 (IGF-1), solute carrier family 2 facilitated glucose transporter member 12 (SLC2a-12), eukaryotic translation initiation factor 4e (EIF-4e), and Unc-51 like autophagy activating kinase 2 (ULK-2)) targeted by at least three altered miRNAs. Predicted upregulation of these mRNA targets were validated by qRT-PCR. Conclusion: This is the first demonstration that sensory neuropathy affects cardiac miRNA expression network targeting IGF-1, SLC2a-12, EIF-4e, and ULK-2, which may contribute to cardiac diastolic dysfunction. These results further support the need for unbiased omics approach followed by in silico prediction and validation of molecular targets to reveal novel pathomechanisms.

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KW - Microrna

KW - Network analysis

KW - Sensory neuropathy

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