Sensory cells of the "rod-" and "cone-type" in the pineal organ of Rana esculenta, as revealed by immunoreaction against opsin and by the presence of an oil (lipid) droplet

B. Vígh, I. Vigh-Teichmann, B. Aros, A. Oksche

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The pineal organ of the frog, Rana esculenta, was studied by use of light- and electron-microscopic methods including immunoreaction against opsin. Most of the morphologically classified cone-type outer segments of the pineal photoreceptors reacted with antisera against opsin of the bovine retina that is dominated by rods. Some of the outer segments of pineal photoreceptor cells remained unstained in accord with the reference tissue, the frog retina, where generally the rods were opsin-positive and most of the cones opsin-negative. The opsin-negative outer segments of pineal photoreceptors were found in continuity with inner segments each containing a large oil (lipid) droplet. These oil droplets stained intensely with osmic acid, Sudan III, Sudan Black B or Scharlach R in cryostat sections, and were soluble in lipid solvents. In ultrathin sections of osmicated material, the oil droplets were homogeneous and of varying electron density. Approximately one tenth of the pineal photoreceptors contained oil droplets and at the same time possessed opsin-immunonegative outer segments. Since in the retina oil droplets and a negative immunoreaction against bovine opsin are characteristic of cones, we suggest that in the pineal organ they also mark "conetype" photoreceptors scattered among "rod-type" photo-receptors, the latter displaying a positive immunoreaction with the antisera used.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalCell and Tissue Research
Volume240
Issue number1
DOIs
Publication statusPublished - Apr 1985

Fingerprint

Rana esculenta
Opsins
Vertebrate Photoreceptor Cells
Cones
Oils
Lipids
Cone Opsins
Retina
sudan III
Anura
Immune Sera
Rod Opsins
Electrons
Osmium Tetroxide
Photoreceptor Cells
Cryostats
Carrier concentration
Lipid Droplets
Tissue
Light

Keywords

  • Cones and rods
  • Oil (lipid) droplets
  • Photoreceptors
  • Pineal organ
  • Rana esculenta (Anura)
  • Rhodopsin

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Sensory cells of the "rod-" and "cone-type" in the pineal organ of Rana esculenta, as revealed by immunoreaction against opsin and by the presence of an oil (lipid) droplet. / Vígh, B.; Vigh-Teichmann, I.; Aros, B.; Oksche, A.

In: Cell and Tissue Research, Vol. 240, No. 1, 04.1985, p. 143-148.

Research output: Contribution to journalArticle

@article{6402c44d9ac94cc6a318677a2f875103,
title = "Sensory cells of the {"}rod-{"} and {"}cone-type{"} in the pineal organ of Rana esculenta, as revealed by immunoreaction against opsin and by the presence of an oil (lipid) droplet",
abstract = "The pineal organ of the frog, Rana esculenta, was studied by use of light- and electron-microscopic methods including immunoreaction against opsin. Most of the morphologically classified cone-type outer segments of the pineal photoreceptors reacted with antisera against opsin of the bovine retina that is dominated by rods. Some of the outer segments of pineal photoreceptor cells remained unstained in accord with the reference tissue, the frog retina, where generally the rods were opsin-positive and most of the cones opsin-negative. The opsin-negative outer segments of pineal photoreceptors were found in continuity with inner segments each containing a large oil (lipid) droplet. These oil droplets stained intensely with osmic acid, Sudan III, Sudan Black B or Scharlach R in cryostat sections, and were soluble in lipid solvents. In ultrathin sections of osmicated material, the oil droplets were homogeneous and of varying electron density. Approximately one tenth of the pineal photoreceptors contained oil droplets and at the same time possessed opsin-immunonegative outer segments. Since in the retina oil droplets and a negative immunoreaction against bovine opsin are characteristic of cones, we suggest that in the pineal organ they also mark {"}conetype{"} photoreceptors scattered among {"}rod-type{"} photo-receptors, the latter displaying a positive immunoreaction with the antisera used.",
keywords = "Cones and rods, Oil (lipid) droplets, Photoreceptors, Pineal organ, Rana esculenta (Anura), Rhodopsin",
author = "B. V{\'i}gh and I. Vigh-Teichmann and B. Aros and A. Oksche",
year = "1985",
month = "4",
doi = "10.1007/BF00217567",
language = "English",
volume = "240",
pages = "143--148",
journal = "Cell and Tissue Research",
issn = "0302-766X",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Sensory cells of the "rod-" and "cone-type" in the pineal organ of Rana esculenta, as revealed by immunoreaction against opsin and by the presence of an oil (lipid) droplet

AU - Vígh, B.

AU - Vigh-Teichmann, I.

AU - Aros, B.

AU - Oksche, A.

PY - 1985/4

Y1 - 1985/4

N2 - The pineal organ of the frog, Rana esculenta, was studied by use of light- and electron-microscopic methods including immunoreaction against opsin. Most of the morphologically classified cone-type outer segments of the pineal photoreceptors reacted with antisera against opsin of the bovine retina that is dominated by rods. Some of the outer segments of pineal photoreceptor cells remained unstained in accord with the reference tissue, the frog retina, where generally the rods were opsin-positive and most of the cones opsin-negative. The opsin-negative outer segments of pineal photoreceptors were found in continuity with inner segments each containing a large oil (lipid) droplet. These oil droplets stained intensely with osmic acid, Sudan III, Sudan Black B or Scharlach R in cryostat sections, and were soluble in lipid solvents. In ultrathin sections of osmicated material, the oil droplets were homogeneous and of varying electron density. Approximately one tenth of the pineal photoreceptors contained oil droplets and at the same time possessed opsin-immunonegative outer segments. Since in the retina oil droplets and a negative immunoreaction against bovine opsin are characteristic of cones, we suggest that in the pineal organ they also mark "conetype" photoreceptors scattered among "rod-type" photo-receptors, the latter displaying a positive immunoreaction with the antisera used.

AB - The pineal organ of the frog, Rana esculenta, was studied by use of light- and electron-microscopic methods including immunoreaction against opsin. Most of the morphologically classified cone-type outer segments of the pineal photoreceptors reacted with antisera against opsin of the bovine retina that is dominated by rods. Some of the outer segments of pineal photoreceptor cells remained unstained in accord with the reference tissue, the frog retina, where generally the rods were opsin-positive and most of the cones opsin-negative. The opsin-negative outer segments of pineal photoreceptors were found in continuity with inner segments each containing a large oil (lipid) droplet. These oil droplets stained intensely with osmic acid, Sudan III, Sudan Black B or Scharlach R in cryostat sections, and were soluble in lipid solvents. In ultrathin sections of osmicated material, the oil droplets were homogeneous and of varying electron density. Approximately one tenth of the pineal photoreceptors contained oil droplets and at the same time possessed opsin-immunonegative outer segments. Since in the retina oil droplets and a negative immunoreaction against bovine opsin are characteristic of cones, we suggest that in the pineal organ they also mark "conetype" photoreceptors scattered among "rod-type" photo-receptors, the latter displaying a positive immunoreaction with the antisera used.

KW - Cones and rods

KW - Oil (lipid) droplets

KW - Photoreceptors

KW - Pineal organ

KW - Rana esculenta (Anura)

KW - Rhodopsin

UR - http://www.scopus.com/inward/record.url?scp=0021906054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021906054&partnerID=8YFLogxK

U2 - 10.1007/BF00217567

DO - 10.1007/BF00217567

M3 - Article

C2 - 3158392

AN - SCOPUS:0021906054

VL - 240

SP - 143

EP - 148

JO - Cell and Tissue Research

JF - Cell and Tissue Research

SN - 0302-766X

IS - 1

ER -