Sensitivity to Glucocorticoids of Lymph Node Cells Stimulated in Vivo by Oxazolone

A. VENETIANER, P. ARÁNYI, Z. Bösze, J. FACHET

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sensitization of B10.A mice with oxazolone results in delayed‐type hypersensitivity (DTH) and a two‐to three‐fold increase in the weight of regional lymph nodes by the third day. Hydrocortisone treatment of the animals 3 days after sensitization suppresses the manifestation of DTH on the tenth day hut not on the seventieth day and significantly decreases the weight of lymph nodes both in control and oxazolone‐sensitized mice. The regional lymph node cells of oxazolone‐stimulated mice were, like the controls, sensitive to dexamethasone in vitro, as judged by viability and thymidine incorporation. Dexamethasone binding capacity of the regional lymph node cells 72 h after oxazolone sensitization increased. However, the dissociation constant for dexamethasone, the binding specificity, and the nuclear transfer of the hormone were not altered. Since high doses of hydrocortisone given.1 days after sensitization were unable to erase permanently the DTH lo oxazolone, it seems very likely that cells responsible for the immunological memory survive the high dose of hydrocortisone.

Original languageEnglish
Pages (from-to)355-362
Number of pages8
JournalScandinavian Journal of Immunology
Volume8
Issue number4
DOIs
Publication statusPublished - 1978

Fingerprint

Oxazolone
Glucocorticoids
Lymph Nodes
Dexamethasone
Hydrocortisone
Hypersensitivity
Immunologic Memory
Weights and Measures
Thymidine
Hormones

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Sensitivity to Glucocorticoids of Lymph Node Cells Stimulated in Vivo by Oxazolone. / VENETIANER, A.; ARÁNYI, P.; Bösze, Z.; FACHET, J.

In: Scandinavian Journal of Immunology, Vol. 8, No. 4, 1978, p. 355-362.

Research output: Contribution to journalArticle

VENETIANER, A. ; ARÁNYI, P. ; Bösze, Z. ; FACHET, J. / Sensitivity to Glucocorticoids of Lymph Node Cells Stimulated in Vivo by Oxazolone. In: Scandinavian Journal of Immunology. 1978 ; Vol. 8, No. 4. pp. 355-362.
@article{5d52cf3f6e9a43019867ef75891a96fd,
title = "Sensitivity to Glucocorticoids of Lymph Node Cells Stimulated in Vivo by Oxazolone",
abstract = "Sensitization of B10.A mice with oxazolone results in delayed‐type hypersensitivity (DTH) and a two‐to three‐fold increase in the weight of regional lymph nodes by the third day. Hydrocortisone treatment of the animals 3 days after sensitization suppresses the manifestation of DTH on the tenth day hut not on the seventieth day and significantly decreases the weight of lymph nodes both in control and oxazolone‐sensitized mice. The regional lymph node cells of oxazolone‐stimulated mice were, like the controls, sensitive to dexamethasone in vitro, as judged by viability and thymidine incorporation. Dexamethasone binding capacity of the regional lymph node cells 72 h after oxazolone sensitization increased. However, the dissociation constant for dexamethasone, the binding specificity, and the nuclear transfer of the hormone were not altered. Since high doses of hydrocortisone given.1 days after sensitization were unable to erase permanently the DTH lo oxazolone, it seems very likely that cells responsible for the immunological memory survive the high dose of hydrocortisone.",
author = "A. VENETIANER and P. AR{\'A}NYI and Z. B{\"o}sze and J. FACHET",
year = "1978",
doi = "10.1111/j.1365-3083.1978.tb00529.x",
language = "English",
volume = "8",
pages = "355--362",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Sensitivity to Glucocorticoids of Lymph Node Cells Stimulated in Vivo by Oxazolone

AU - VENETIANER, A.

AU - ARÁNYI, P.

AU - Bösze, Z.

AU - FACHET, J.

PY - 1978

Y1 - 1978

N2 - Sensitization of B10.A mice with oxazolone results in delayed‐type hypersensitivity (DTH) and a two‐to three‐fold increase in the weight of regional lymph nodes by the third day. Hydrocortisone treatment of the animals 3 days after sensitization suppresses the manifestation of DTH on the tenth day hut not on the seventieth day and significantly decreases the weight of lymph nodes both in control and oxazolone‐sensitized mice. The regional lymph node cells of oxazolone‐stimulated mice were, like the controls, sensitive to dexamethasone in vitro, as judged by viability and thymidine incorporation. Dexamethasone binding capacity of the regional lymph node cells 72 h after oxazolone sensitization increased. However, the dissociation constant for dexamethasone, the binding specificity, and the nuclear transfer of the hormone were not altered. Since high doses of hydrocortisone given.1 days after sensitization were unable to erase permanently the DTH lo oxazolone, it seems very likely that cells responsible for the immunological memory survive the high dose of hydrocortisone.

AB - Sensitization of B10.A mice with oxazolone results in delayed‐type hypersensitivity (DTH) and a two‐to three‐fold increase in the weight of regional lymph nodes by the third day. Hydrocortisone treatment of the animals 3 days after sensitization suppresses the manifestation of DTH on the tenth day hut not on the seventieth day and significantly decreases the weight of lymph nodes both in control and oxazolone‐sensitized mice. The regional lymph node cells of oxazolone‐stimulated mice were, like the controls, sensitive to dexamethasone in vitro, as judged by viability and thymidine incorporation. Dexamethasone binding capacity of the regional lymph node cells 72 h after oxazolone sensitization increased. However, the dissociation constant for dexamethasone, the binding specificity, and the nuclear transfer of the hormone were not altered. Since high doses of hydrocortisone given.1 days after sensitization were unable to erase permanently the DTH lo oxazolone, it seems very likely that cells responsible for the immunological memory survive the high dose of hydrocortisone.

UR - http://www.scopus.com/inward/record.url?scp=0018134657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018134657&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3083.1978.tb00529.x

DO - 10.1111/j.1365-3083.1978.tb00529.x

M3 - Article

C2 - 715380

AN - SCOPUS:0018134657

VL - 8

SP - 355

EP - 362

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 4

ER -