Sensitivity of redox reactions of dyes to variations of conditions created in mass spectrometric experiments

Marina V. Kosevich, Vitalij V. Chagovets, Irina V. Shmigol, Sergej V. Snegir, Oleg A. Boryak, Vadim V. Orlov, Vadim S. Shelkovsky, Valerij A. Pokrovskiy, Agnes Gomory

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Redox behaviour of four imidazophenazine dye derivatives under mass spectrometric conditions of matrix-assisted laser desorption/ionization (MALDI), laser desorption/ionization (LDI) from metal and graphite surface, electrospray, low temperature secondary ion mass spectrometry (LT SIMS) and fast atom bombardment (FAB) was studied and distinctions in the reduction-dependent spectral patterns were analyzed from the point of view of different quantities of protons and electrons available for reduction in different techniques. The reduction products [M + 2H]+•, [M+3H]+ and M -•, [M+H]- were observed in the positive and negative ion modes, respectively, which permitted to suggest independent occurrence of reduction and protonation/deprotonation processes. LDI from graphite substrate was the only technique that allowed us to obtain abundant negative ions of all dye derivatives. The yield of field ionization (FI) or field desorption (FD) mechanism to ion formation under LDI from rough graphite surface has been addressed. The sensitivity of reduction of the dyes to variation of reduction-initiating agents confirms high redox activity of the dyes essential for their functioning in natural and artificial systems.

Original languageEnglish
Pages (from-to)1402-1412
Number of pages11
JournalJournal of Mass Spectrometry
Issue number10
Publication statusPublished - Oct 1 2008



  • Electron affinity
  • Electrospray
  • Imidazophenazine dyes
  • Mass spectrometry
  • Redox reactions

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Kosevich, M. V., Chagovets, V. V., Shmigol, I. V., Snegir, S. V., Boryak, O. A., Orlov, V. V., Shelkovsky, V. S., Pokrovskiy, V. A., & Gomory, A. (2008). Sensitivity of redox reactions of dyes to variations of conditions created in mass spectrometric experiments. Journal of Mass Spectrometry, 43(10), 1402-1412.