Sensitivity enhancement of a "bananatrode" biosensor for dopamine based on SECM studies inside its reaction layer

Zsuzsanna Ori, András Kiss, Anton Alexandru Ciucu, Constantin Mihailciuc, Cristian Dragos Stefanescu, Livia Nagy, Géza Nagy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A model biosensor based on a reaction layer containing banana pulp was constructed and dopamine (DA) has been selected as analyte for the investigations. On the basis of dynamic voltammograms it was found that the optimal potential for DA detection should be -0.2 V vs. SCE, potential where the oxygen interference was avoided. Based on vertical scanning electrochemical microscopy (SECM) the optimal width of the reaction layer for the model biosensor was determined to be in between 130 and 160 μm. Optimal resting potential in periodically interrupted amperometry (PIA) detection mode was determined to be in the range from -0.02 V to -0.03 V vs. SCE. The resting time used in PIA experiments was 0.5 s. A detection limit of 2 × 10 -6 M was obtained for DA by using PIA detection mode.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume190
DOIs
Publication statusPublished - Jan 1 2014

Keywords

  • Banana tissue biosensor
  • Dopamine
  • Dynamic voltammetry
  • Periodically interrupted amperometry
  • Scanning electrochemical microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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