Sensitivity and specificity of immunohistological methods on freeze-dried paraffin sections

Z. Nemes, V. Thomázy, G. Szeifert

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The application of freeze-dried paraffin sections for immunohistology eliminates many of the problems associated with the cryostat technique: the sections are thin sufficiently, the cell morphology is improved, there are no diffusion artifacts and the intensity of immunohistological reactions is superior to that obtained on cryostat sections. The preservation of antogenicity in freeze-dried paraffin sections is sufficient for the demonstration of light chain monotypia in lymphomas of B-cell origin. Indirect immunofluorescence is the method of choice for demonstrating surface Ig in freeze-dried paraffin sections.

Original languageEnglish
Pages (from-to)53-64
Number of pages12
JournalJournal of Immunological Methods
Volume49
Issue number1
DOIs
Publication statusPublished - Feb 26 1982

Fingerprint

Paraffin
Sensitivity and Specificity
B-Cell Lymphoma
Indirect Fluorescent Antibody Technique
Artifacts
Light

Keywords

  • freeze-dried paraffin sections
  • immunohistology
  • light chain monotypia

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Sensitivity and specificity of immunohistological methods on freeze-dried paraffin sections. / Nemes, Z.; Thomázy, V.; Szeifert, G.

In: Journal of Immunological Methods, Vol. 49, No. 1, 26.02.1982, p. 53-64.

Research output: Contribution to journalArticle

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