Sensitive method for glycosaminoglycan analysis of tissue sections

Lilla Turiák, Gábor Tóth, Oliver Ozohanics, Ágnes Révész, András Ács, K. Vékey, Joseph Zaia, L. Drahos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC–MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10 μg).

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalJournal of Chromatography A
Volume1544
DOIs
Publication statusPublished - Apr 6 2018

Fingerprint

Disaccharides
Glycosaminoglycans
A73025
Tissue
Limit of Detection
Digestion
Mass Spectrometry
Prostatic Neoplasms
Microarrays
High Pressure Liquid Chromatography
Ionization
Mass spectrometry
Sensitivity and Specificity
Neoplasms

Keywords

  • Glycosaminoglycan
  • Heparan sulfate
  • Hydrophilic interaction chromatography
  • Mass spectrometry
  • Prostate cancer
  • Tissue microarray

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Sensitive method for glycosaminoglycan analysis of tissue sections. / Turiák, Lilla; Tóth, Gábor; Ozohanics, Oliver; Révész, Ágnes; Ács, András; Vékey, K.; Zaia, Joseph; Drahos, L.

In: Journal of Chromatography A, Vol. 1544, 06.04.2018, p. 41-48.

Research output: Contribution to journalArticle

Turiák, Lilla ; Tóth, Gábor ; Ozohanics, Oliver ; Révész, Ágnes ; Ács, András ; Vékey, K. ; Zaia, Joseph ; Drahos, L. / Sensitive method for glycosaminoglycan analysis of tissue sections. In: Journal of Chromatography A. 2018 ; Vol. 1544. pp. 41-48.
@article{48dd1f388db6434db294a24cf410a359,
title = "Sensitive method for glycosaminoglycan analysis of tissue sections",
abstract = "A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC–MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10 μg).",
keywords = "Glycosaminoglycan, Heparan sulfate, Hydrophilic interaction chromatography, Mass spectrometry, Prostate cancer, Tissue microarray",
author = "Lilla Turi{\'a}k and G{\'a}bor T{\'o}th and Oliver Ozohanics and {\'A}gnes R{\'e}v{\'e}sz and Andr{\'a}s {\'A}cs and K. V{\'e}key and Joseph Zaia and L. Drahos",
year = "2018",
month = "4",
day = "6",
doi = "10.1016/j.chroma.2018.02.034",
language = "English",
volume = "1544",
pages = "41--48",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",

}

TY - JOUR

T1 - Sensitive method for glycosaminoglycan analysis of tissue sections

AU - Turiák, Lilla

AU - Tóth, Gábor

AU - Ozohanics, Oliver

AU - Révész, Ágnes

AU - Ács, András

AU - Vékey, K.

AU - Zaia, Joseph

AU - Drahos, L.

PY - 2018/4/6

Y1 - 2018/4/6

N2 - A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC–MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10 μg).

AB - A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC–MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10 μg).

KW - Glycosaminoglycan

KW - Heparan sulfate

KW - Hydrophilic interaction chromatography

KW - Mass spectrometry

KW - Prostate cancer

KW - Tissue microarray

UR - http://www.scopus.com/inward/record.url?scp=85042667234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042667234&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2018.02.034

DO - 10.1016/j.chroma.2018.02.034

M3 - Article

C2 - 29506752

AN - SCOPUS:85042667234

VL - 1544

SP - 41

EP - 48

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

ER -