Sensitive method for glycosaminoglycan analysis of tissue sections

Lilla Turiák, Gábor Tóth, Oliver Ozohanics, Ágnes Révész, András Ács, Károly Vékey, Joseph Zaia, László Drahos

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A simple, isocratic HPLC method based on HILIC-WAX separation, has been developed for analyzing sulfated disaccharides of glycosaminoglycans (GAGs). To our best knowledge, this is the first successful attempt using this special phase in nano-HPLC–MS analysis. Mass spectrometry was based on negative ionization, improving both sensitivity and specificity. Detection limit for most sulfated disaccharides were approximately 1 fmol; quantitation limits 10 fmol. The method was applied for glycosaminoglycan profiling of tissue samples, using surface digestion protocols. This novel combination provides sufficient sensitivity for GAG disaccharide analysis, which was first performed using prostate cancer tissue microarrays. Preliminary results show that GAG analysis may be useful for identifying cancer related changes in small amounts of tissue samples (ca. 10 μg).

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalJournal of Chromatography A
Volume1544
DOIs
Publication statusPublished - Apr 6 2018

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Keywords

  • Glycosaminoglycan
  • Heparan sulfate
  • Hydrophilic interaction chromatography
  • Mass spectrometry
  • Prostate cancer
  • Tissue microarray

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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