Sensitive ELISA Test for Determination of Ochratoxin A

Ildikó Barna-Vetró, László Solti, József Téren, Ágnes Gyöngyösi, Erzsébet Szabó, Anna Wolfling

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A direct, competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody has been developed for quantitative determination of ochratoxin A (OA) in different cereals. A dichloromethane/citric acid mixture was used for extraction of cereals. This cleanup procedure proved to be as effective for ochratoxin A extraction as protocols using strong acids. The mean withinassay and interassay coefficients of variation for the standard curve was <10%. The range of this test is 1-10 ng/g, with a detection limit of 0.5 ng/g OA. The toxin recovery from cereals infected with 5-100 ng/mL OA varied between 90 and 130%.

Original languageEnglish
Pages (from-to)4071-4074
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume44
Issue number12
DOIs
Publication statusPublished - Dec 1996

    Fingerprint

Keywords

  • Cereals
  • ELISA
  • Ochratoxin A

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this