Sensing photosynthetic herbicides in an electrochemical flow cell

Tibor Szabó, Richárd Csekő, Kata Hajdu, Krisztina Nagy, Orsolya Sipos, Péter Galajda, Győző Garab, László Nagy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Specific inhibitory reactions of herbicides with photosynthetic reaction centers bound to working electrodes were monitored in a conventional electrochemical cell and a newly designed microfluidic electrochemical flow cell. In both cases, the bacterial reaction centers were bound to a transparent conductive metal oxide, indium-tin-oxide, electrode through carbon nanotubes. In the conventional cell, photocurrent densities of up to a few μA/cm2 could be measured routinely. The photocurrent could be blocked by the photosynthetic inhibitor terbutryn (I50 = 0.38 ± 0.14 μM) and o-phenanthroline (I50 = 63.9 ± 12.2 μM). The microfluidic flow cell device enabled us to reduce the sample volume and to simplify the electrode arrangement. The useful area of the electrodes remained the same (ca. 2 cm2), similar to the classical electrochemical cell; however, the size of the cell was reduced considerably. The microfluidic flow control enabled us monitoring in real time the binding/unbinding of the inhibitor and cofactor molecules at the secondary quinone site.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalPhotosynthesis research
Volume132
Issue number2
DOIs
Publication statusPublished - May 1 2017

Keywords

  • Biocomposites
  • Bioelectronics
  • Herbicide biosensors
  • Photocurrent
  • Photosynthesis

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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