Sensing hydrogen peroxide by carbon nanotube/horseradish peroxidase bio-nanocomposite

Melinda Magyar, Kata Hajdu, Tibor Szabó, Balázs Endrodi, Klára Hernádi, Endre Horváth, Arnaud Magrez, László Forró, Csaba Visy, László Nagy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

H2O2 is a product of reactions catalysed by several oxidase enzymes and it is essential in environmental and pharmaceutical analyses. The most commonly used enzyme in understanding the biological behaviour of catalysed oxidation of H2O2 is horseradish peroxidase (HRP). In our experiments HRP was bound to carboxyl-functionalized multiwalled carbon nanotubes (MWNT-COOH) by N-hydroxysuccinimide (NHS) and 1-[3-dimethylaminopropyl]-3-ethyl-carbodiimide (EDC) crosslinkers. The activity of this bio-nanocomposite and the limit of detection (LOD) for H2O2 were determined by measuring the fluorescence of tetraguaiacol (which chemical is the product of guaiacol oxidation after addition of H2O2 to the reaction mixture) as a function of time. The hydrogen peroxide biosensor we developed exhibited a detection limit of 1.2μMH2O2s-1 which resolution was better than the one measured in solution by about a factor of eight (it was 10μMH2O2s-1 in solution). An attempt has been made to measure the concentration of H2O2 in an electrochemical cell with HRP immobilized on the surface of an electrode made of indium tin oxide (ITO, a transparent conductive oxide) and MWNT.

Original languageEnglish
Pages (from-to)2559-2563
Number of pages5
JournalPhysica Status Solidi (B) Basic Research
Volume250
Issue number12
DOIs
Publication statusPublished - Dec 1 2013

    Fingerprint

Keywords

  • Carbon nanotube
  • Horseradish peroxidase
  • ITO

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Magyar, M., Hajdu, K., Szabó, T., Endrodi, B., Hernádi, K., Horváth, E., Magrez, A., Forró, L., Visy, C., & Nagy, L. (2013). Sensing hydrogen peroxide by carbon nanotube/horseradish peroxidase bio-nanocomposite. Physica Status Solidi (B) Basic Research, 250(12), 2559-2563. https://doi.org/10.1002/pssb.201300079