Semiquantitative evaluation of histochemically detectable calcium binding in mammalian brain slices

A. Mihály, U. Kuhnt, L. Siklós, F. Joó

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A method is described for the electron histochemical demonstration of Ca in the central nervous system, based on fixation in Ca-containing paraformaldehyde solution and the subsequent complexing of Ca by ammonium oxalate. The method resulted in highly electron-dense deposits, with good ultrastructural preservation. The Ca content of the deposits was proved by physico-chemical analysis. The high electron density permitted the counting of deposits and thereby an estimation of their numerical density, via planimetry of electron micrographs. Since pre- and postsynaptic localizations could be distinguished on the basis of ultrastructure, this procedure is regarded as a unique semiquantitative method for estimation of the tissue Ca binding of mammalian brain slices.

Original languageEnglish
Pages (from-to)283-293
Number of pages11
JournalJournal of Neuroscience Methods
Volume20
Issue number4
DOIs
Publication statusPublished - 1987

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Electrons
Calcium
Brain
Oxalic Acid
Central Nervous System
paraform

Keywords

  • Brain slice
  • Calcium
  • Histochemistry
  • Ultrastructure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Semiquantitative evaluation of histochemically detectable calcium binding in mammalian brain slices. / Mihály, A.; Kuhnt, U.; Siklós, L.; Joó, F.

In: Journal of Neuroscience Methods, Vol. 20, No. 4, 1987, p. 283-293.

Research output: Contribution to journalArticle

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