Self transglutaminase-based rapid coeliac disease antibody detection by a lateral flow method

T. Raivio, K. Kaukinen, É Nemes, K. Laurila, P. Collin, J. B. Kovács, M. Mäki, I. R. Korponay-Szabó

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Abstract

Background: The conventional coeliac disease antibody tests require patient's sera, and are laborious and time-consuming. Aim: To evaluate a newly developed rapid whole blood test in coeliac disease antibody detection, and its suitability for office use. Methods: Endogenous tissue transglutaminase found in red blood cells in a whole blood fingertip or venous sample is liberated upon haemolysis and complexes with tissue transglutaminase antibodies, if present. The complexes, captured by a lateral flow system, are visualized within 5 min. Stored samples from 121 untreated, 106 treated coeliac disease patients and 107 controls were evaluated and compared with serum endomysium and tissue transglutaminase antibody tests and histology; 150 patients were prospectively tested on site in the doctor's office. Results: The rapid test showed sensitivity (96.7%) comparable with the serum endomysium and tissue transglutaminase antibody tests from stored samples; specificity was slightly lower (93.5%). When tested on site the results were concordant in 96.7% of cases compared with endomysium and tissue transglutaminase antibody results. The test recognized the disappearance of tissue transglutaminase antibodies on a gluten-free diet. Conclusions: The self tissue transglutaminase-based rapid test can be easily carried out from a fingertip blood sample on site in the physician's office for both coeliac disease case finding and dietary monitoring purposes.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalAlimentary Pharmacology and Therapeutics
Volume24
Issue number1
DOIs
Publication statusPublished - Jul 1 2006

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ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology
  • Pharmacology (medical)

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