Self-similarity of Mn(II)-induced trypsin activity oscillations. Experimental evidence

Tamás Vajda, Ferenc Almási, T. Vántus

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aperiodic self-similar oscillations of trypsin activity were shown by experiments conducted in the presence of Mn2+ ion at pH 8.2 and -10°C in frozen (cryo-oscillations) and at 0° and 25° C under unstirred conditions, respectively. The solution of trypsin obtained by trypsinogen activation and of 0.1 M MnCl2 was distributed into samples or kept in batches at the experimental temperature and sampled. At given time intervals the samples were tested for tryptic activity. In experiments at -10° C the samples were frozen at the initiating of the series. The irregular shapes of activity curves as well as the trajectory of a next-amplitude plot is discussed as a result of a sensitive coupling between the chemical/conformational and diffusional controls of the enzyme activity in the far-from-equilibrium heterogeneous system. These indicate that the self-similar character of the trypsin activity oscillations can be considered on a phenomenological level.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalJournal of Inorganic Biochemistry
Volume52
Issue number2
DOIs
Publication statusPublished - Nov 1 1993

Fingerprint

Trypsin
Trypsinogen
Enzyme activity
Experiments
Chemical activation
Trajectories
Ions
Temperature
Enzymes
manganese chloride

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Self-similarity of Mn(II)-induced trypsin activity oscillations. Experimental evidence. / Vajda, Tamás; Almási, Ferenc; Vántus, T.

In: Journal of Inorganic Biochemistry, Vol. 52, No. 2, 01.11.1993, p. 131-138.

Research output: Contribution to journalArticle

@article{87e1a3ca62894c688cca9439b23abedf,
title = "Self-similarity of Mn(II)-induced trypsin activity oscillations. Experimental evidence",
abstract = "Aperiodic self-similar oscillations of trypsin activity were shown by experiments conducted in the presence of Mn2+ ion at pH 8.2 and -10°C in frozen (cryo-oscillations) and at 0° and 25° C under unstirred conditions, respectively. The solution of trypsin obtained by trypsinogen activation and of 0.1 M MnCl2 was distributed into samples or kept in batches at the experimental temperature and sampled. At given time intervals the samples were tested for tryptic activity. In experiments at -10° C the samples were frozen at the initiating of the series. The irregular shapes of activity curves as well as the trajectory of a next-amplitude plot is discussed as a result of a sensitive coupling between the chemical/conformational and diffusional controls of the enzyme activity in the far-from-equilibrium heterogeneous system. These indicate that the self-similar character of the trypsin activity oscillations can be considered on a phenomenological level.",
author = "Tam{\'a}s Vajda and Ferenc Alm{\'a}si and T. V{\'a}ntus",
year = "1993",
month = "11",
day = "1",
doi = "10.1016/0162-0134(93)85029-8",
language = "English",
volume = "52",
pages = "131--138",
journal = "Journal of Inorganic Biochemistry",
issn = "0162-0134",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Self-similarity of Mn(II)-induced trypsin activity oscillations. Experimental evidence

AU - Vajda, Tamás

AU - Almási, Ferenc

AU - Vántus, T.

PY - 1993/11/1

Y1 - 1993/11/1

N2 - Aperiodic self-similar oscillations of trypsin activity were shown by experiments conducted in the presence of Mn2+ ion at pH 8.2 and -10°C in frozen (cryo-oscillations) and at 0° and 25° C under unstirred conditions, respectively. The solution of trypsin obtained by trypsinogen activation and of 0.1 M MnCl2 was distributed into samples or kept in batches at the experimental temperature and sampled. At given time intervals the samples were tested for tryptic activity. In experiments at -10° C the samples were frozen at the initiating of the series. The irregular shapes of activity curves as well as the trajectory of a next-amplitude plot is discussed as a result of a sensitive coupling between the chemical/conformational and diffusional controls of the enzyme activity in the far-from-equilibrium heterogeneous system. These indicate that the self-similar character of the trypsin activity oscillations can be considered on a phenomenological level.

AB - Aperiodic self-similar oscillations of trypsin activity were shown by experiments conducted in the presence of Mn2+ ion at pH 8.2 and -10°C in frozen (cryo-oscillations) and at 0° and 25° C under unstirred conditions, respectively. The solution of trypsin obtained by trypsinogen activation and of 0.1 M MnCl2 was distributed into samples or kept in batches at the experimental temperature and sampled. At given time intervals the samples were tested for tryptic activity. In experiments at -10° C the samples were frozen at the initiating of the series. The irregular shapes of activity curves as well as the trajectory of a next-amplitude plot is discussed as a result of a sensitive coupling between the chemical/conformational and diffusional controls of the enzyme activity in the far-from-equilibrium heterogeneous system. These indicate that the self-similar character of the trypsin activity oscillations can be considered on a phenomenological level.

UR - http://www.scopus.com/inward/record.url?scp=0027383890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027383890&partnerID=8YFLogxK

U2 - 10.1016/0162-0134(93)85029-8

DO - 10.1016/0162-0134(93)85029-8

M3 - Article

VL - 52

SP - 131

EP - 138

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

SN - 0162-0134

IS - 2

ER -