Self-similarity of Mn(II)-induced trypsin activity oscillations. Experimental evidence

Tamás Vajda, Ferenc Almási, Tiber Vántus

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Abstract

Aperiodic self-similar oscillations of trypsin activity were shown by experiments conducted in the presence of Mn2+ ion at pH 8.2 and -10°C in frozen (cryo-oscillations) and at 0° and 25° C under unstirred conditions, respectively. The solution of trypsin obtained by trypsinogen activation and of 0.1 M MnCl2 was distributed into samples or kept in batches at the experimental temperature and sampled. At given time intervals the samples were tested for tryptic activity. In experiments at -10° C the samples were frozen at the initiating of the series. The irregular shapes of activity curves as well as the trajectory of a next-amplitude plot is discussed as a result of a sensitive coupling between the chemical/conformational and diffusional controls of the enzyme activity in the far-from-equilibrium heterogeneous system. These indicate that the self-similar character of the trypsin activity oscillations can be considered on a phenomenological level.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalJournal of Inorganic Biochemistry
Volume52
Issue number2
DOIs
Publication statusPublished - Nov 1 1993

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ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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