Self-crowding influences the temperature – pressure stability of the human telomere G-quadruplex

J. Somkuti, M. Adányi, L. Smeller

Research output: Contribution to journalArticle

Abstract

We measured the effect of crowded environment on G-quadruplex structures, formed by guanine rich DNA sequences. Fluorescence and infrared spectroscopy were used to determine the temperature stability of G-quadruplex structure formed by the human telomere sequence. We determined the T-p phase diagram of Htel aptamer up to 1 GPa at different self-crowding conditions. The unfolding volume change was determined from the pressure induced shift of the unfolding temperature of the quadruplex form. The unfolding volume change decreased in magnitude, and even its sign changed from negative (−19 ml/mol) to positive (7 ml/mol) under self-crowded conditions. The possible explanations are the appearance of the parallel GQ structure at high concentration or the fact that the volume decrease caused by the released central K+ ion during the unfolding is less significant in crowded environment.

Original languageEnglish
Article number106248
JournalBiophysical Chemistry
Volume254
DOIs
Publication statusPublished - Nov 1 2019

Fingerprint

G-Quadruplexes
Crowding
Telomere
Pressure
Temperature
DNA sequences
Fluorescence Spectrometry
Fluorescence spectroscopy
Guanine
Phase diagrams
Infrared spectroscopy
Ions

Keywords

  • Crowding
  • FRET
  • FTIR
  • Htel
  • Pressure

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

Cite this

Self-crowding influences the temperature – pressure stability of the human telomere G-quadruplex. / Somkuti, J.; Adányi, M.; Smeller, L.

In: Biophysical Chemistry, Vol. 254, 106248, 01.11.2019.

Research output: Contribution to journalArticle

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