Selenium speciation in Agaricus bisporus and Lentinula edodes mushroom proteins using multi-dimensional chromatography coupled to inductively coupled plasma mass spectrometry

Valéria Gergely, Kevin M. Kubachka, Sandra Mounicou, P. Fodor, Joseph A. Caruso

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

In this study, selenium species from Se containing proteins in mushrooms (Agaricus bisporus and Lentinula edodes) were investigated with size-exclusion liquid chromatography coupled to UV and inductively coupled plasma mass spectrometry (ICP-MS). Different protein extraction protocols were investigated. Variability of the fractionation patterns with three extraction media (0.1 M NaOH, 30 mM Tris-HCl, and enzymatic digestions) was evaluated for both mushroom types. A 24 h Tris-HCl extraction followed by acetone addition was found to be optimal for protein precipitation. Presumably protein bound selenoamino acids were released using enzymes (proteinase K, protease XIV and trypsin). The selenium speciation of the proteolytic extract of the water soluble proteins fraction was carried out by using reversed-phase ion-pairing high performance liquid chromatography (RP-HPIPC) coupled on-line to ICP-MS for selenium specific detection. Selenocystine, selenomethionine, methylselenocysteine and inorganic selenium were established in both samples utilizing retention time standards and standard additions to the sample.

Original languageEnglish
Pages (from-to)94-102
Number of pages9
JournalJournal of Chromatography A
Volume1101
Issue number1-2
DOIs
Publication statusPublished - Jan 6 2006

Fingerprint

Shiitake Mushrooms
Agaricus
Inductively coupled plasma mass spectrometry
Agaricales
Selenium
Chromatography
Mass Spectrometry
Proteins
Selenomethionine
Endopeptidase K
Pronase
Size exclusion chromatography
Liquid chromatography
High performance liquid chromatography
Fractionation
Acetone
Liquid Chromatography
Trypsin
Gel Chromatography
Digestion

Keywords

  • HPLC-ICP-MS
  • Mushroom
  • Proteins
  • Selenium speciation

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Selenium speciation in Agaricus bisporus and Lentinula edodes mushroom proteins using multi-dimensional chromatography coupled to inductively coupled plasma mass spectrometry. / Gergely, Valéria; Kubachka, Kevin M.; Mounicou, Sandra; Fodor, P.; Caruso, Joseph A.

In: Journal of Chromatography A, Vol. 1101, No. 1-2, 06.01.2006, p. 94-102.

Research output: Contribution to journalArticle

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