Selenite-stress selected mutant strains of probiotic bacteria for Se source production

T. Pusztahelyi, Szilvia Kovács, I. Pócsi, József Prokisch

Research output: Contribution to journalArticle

12 Citations (Scopus)


Selenium deficiency is a major health problem worldwide for about 1 billion people. Bacterial cells usually possess low tolerance to selenite stress and also low ability to reduce high concentrations of toxic selenite. Here, high tolerance to selenite and selenium bioaccumulation capability were developed in mutated clones of probiotic and starter bacteria including Enterococcus faecium, Bifidobacterium animalis ssp. lactis, Lactobacillus casei and Lactococcus lactis ssp. lactis by food-level strain development process and clone selection. All mutant clones possessed increased glutathione concentration and glutathione reductase activity. The selenite treatment increased further these values in L. casei mutant strain pointing at a different selenite reduction pathway and/or stress response in this organism. Considerable conversion of selenite to cell bound selenium forms with a concomitant high biomass production was detected in E. faecium and B. animalis ssp. lactis cultures. Possible application of these strains as food and feed supplements is under investigation.

Original languageEnglish
Pages (from-to)96-101
Number of pages6
JournalJournal of Trace Elements in Medicine and Biology
Publication statusPublished - Apr 1 2015



  • Glutathione
  • Glutathione reductase
  • Lactic acid bacteria
  • Selenite
  • Selenium

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry
  • Molecular Medicine

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