An anucleate small-protoplast fraction was prepared from a respiratory-competent Saccharomyces cerevisiae strain carrying mitochondrially inherited resistance to erythromycin, and used to transfer mitochondria selectively. Polyethylene glycol and Ca2+ were applied to induce fusion between these small protoplasts and nucleus-containing protoplasts of a respiratory-deficient ρ° mutant derived from an adenine-requiring strain of the same species. The majority of fusion products were haploid and erythromycin resistant, containing the nucleus of the recipient adenine-requiring strain and the mitochondrial genome from the respiratory-competent donor cells. Selective transfer of mitochondria and other cytoplasmic genetic elements also seems possible in a wide variety of fungal and other cells.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology