Selective transfer of fungal cytoplasmic genetic elements by protoplast fusion

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9 Citations (Scopus)

Abstract

An anucleate small-protoplast fraction was prepared from a respiratory-competent Saccharomyces cerevisiae strain carrying mitochondrially inherited resistance to erythromycin, and used to transfer mitochondria selectively. Polyethylene glycol and Ca2+ were applied to induce fusion between these small protoplasts and nucleus-containing protoplasts of a respiratory-deficient ρ° mutant derived from an adenine-requiring strain of the same species. The majority of fusion products were haploid and erythromycin resistant, containing the nucleus of the recipient adenine-requiring strain and the mitochondrial genome from the respiratory-competent donor cells. Selective transfer of mitochondria and other cytoplasmic genetic elements also seems possible in a wide variety of fungal and other cells.

Original languageEnglish
Pages (from-to)343-345
Number of pages3
JournalCurrent Microbiology: An International Journal
Volume4
Issue number6
DOIs
Publication statusPublished - Nov 1980

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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