Selective TGF-β1/ALK inhibitor improves neuronal differentiation of mouse embryonic stem cells

Nuttha Klincumhom, Theerawat Tharasanit, Chommanart Thongkittidilok, Narong Tiptanavattana, Sasitorn Rungarunlert, A. Dinnyés, Mongkol Techakumphu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The transforming growth factor-β1 (TGF-β1), a polypeptide member of the TGF-β superfamily, has myriad cellular functions, including cell fate differentiation. We hypothesized that suppression of TGF-β1 signaling would improve the efficacy of neuronal differentiation during embryoid body (EB) development. In this study, mouse embryonic stem cells (ESCs) were allowed to differentiate into their neuronal lineage, both with, and without the TGF-β1 inhibitor (A83-01). After 8 days of EB suspension culture, the samples were examined by morphological analysis, immunocytochemistry and immunohistochemistry with pluripotent (Oct4, Sox2) and neuronal specific markers (Pax6, NeuN). The alteration of gene expressions during EB development was determined by quantitative RT-PCR. Our results revealed that the TGF-β1/ALK inhibitor potentially suppressed pluripotent gene (Oct4) during a rapidly up-regulation of neuronal associated genes including Sox1 and MAP2. Strikingly, during EB development, the expression of GFAP, the astrocyte specific gene, remarkably decreased compared to the non-treated control. This strategy demonstrated the beneficial function of TGF-β1/ALK inhibitor that rapidly and uniformly drives cell fate alteration from pluripotent state toward neuronal lineages.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalNeuroscience Letters
Volume578
DOIs
Publication statusPublished - Aug 22 2014

Fingerprint

Transforming Growth Factors
Embryoid Bodies
Immunohistochemistry
Genes
Astrocytes
Cell Differentiation
Suspensions
Up-Regulation
Mouse Embryonic Stem Cells
Gene Expression
Polymerase Chain Reaction
Peptides

Keywords

  • Embryonic stem cells
  • Mouse
  • Neuronal differentiation
  • TGF-β1/ALK inhibitor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Klincumhom, N., Tharasanit, T., Thongkittidilok, C., Tiptanavattana, N., Rungarunlert, S., Dinnyés, A., & Techakumphu, M. (2014). Selective TGF-β1/ALK inhibitor improves neuronal differentiation of mouse embryonic stem cells. Neuroscience Letters, 578, 1-6. https://doi.org/10.1016/j.neulet.2014.06.001

Selective TGF-β1/ALK inhibitor improves neuronal differentiation of mouse embryonic stem cells. / Klincumhom, Nuttha; Tharasanit, Theerawat; Thongkittidilok, Chommanart; Tiptanavattana, Narong; Rungarunlert, Sasitorn; Dinnyés, A.; Techakumphu, Mongkol.

In: Neuroscience Letters, Vol. 578, 22.08.2014, p. 1-6.

Research output: Contribution to journalArticle

Klincumhom, N, Tharasanit, T, Thongkittidilok, C, Tiptanavattana, N, Rungarunlert, S, Dinnyés, A & Techakumphu, M 2014, 'Selective TGF-β1/ALK inhibitor improves neuronal differentiation of mouse embryonic stem cells', Neuroscience Letters, vol. 578, pp. 1-6. https://doi.org/10.1016/j.neulet.2014.06.001
Klincumhom, Nuttha ; Tharasanit, Theerawat ; Thongkittidilok, Chommanart ; Tiptanavattana, Narong ; Rungarunlert, Sasitorn ; Dinnyés, A. ; Techakumphu, Mongkol. / Selective TGF-β1/ALK inhibitor improves neuronal differentiation of mouse embryonic stem cells. In: Neuroscience Letters. 2014 ; Vol. 578. pp. 1-6.
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