Selective suppression of an endothelin and platelet-derived growth factor containing vesicular system in endothelium of rat saphenous vein by long-term orthostasis

Gábor Raffai, E. Fehér, G. Nádasy, S. Paku, Gábor Pogány, Ferenc Tímár, B. Szende, E. Monos

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Abstract

Electron-dense vesicles were observed in rat vascular endothelium. The purpose of this study was to characterize their content(s), venous-arterial distribution and response to chronic orthostatic stress in extremity vessels. Saphenous and brachial vessels - saphenous vein (SV), saphenous artery (SA), brachial vein, brachial artery - were prepared for electron microscopy to quantitate the vesicle area within the endothelium following immunohistochemical and immunocytochemical identification. The effect of long-term orthostasis was assessed by exposure to head-up tilt for 2 weeks. The vesicular area in relation to the total cross-sectional area of the endothelial cells in the SV and SA of normal and confined control groups was 3.88 ± 0.38 versus 0.89 ± 0.06% (p <0.05) and 4.92 ± 0.25 versus 1.09 ± 0.47% (p <0.05), respectively. Head-up tilt suppressed the vesicle content of the SV to 2.26 ± 0.39% (p <0.05), but it remained low in the SA (1.29 ± 0.45%), brachial vein (0.45 ± 0.12%) and brachial artery (0.59 ± 0.17%). Endothelin and platelet-derived growth factor, but not acidic phosphatase activity or lipid content, could be identified in the vesicles. Plasma endothelin levels were unchanged. We conclude that dense vesicles in the endothelium of extremity vessels are not cell degradation products. They may represent a vesicular secretory or storage system for endothelin and platelet-derived growth factor which participates in regional vascular adaptation to long-term orthostatic load.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalJournal of Vascular Research
Volume42
Issue number2
DOIs
Publication statusPublished - Mar 2005

Fingerprint

Brachial Artery
Platelet-Derived Growth Factor
Endothelins
Saphenous Vein
Dizziness
Endothelium
Veins
Arm
Extremities
Arteries
Head
Vascular Endothelium
Phosphoric Monoester Hydrolases
Blood Vessels
Electron Microscopy
Endothelial Cells
Electrons
Lipids
Control Groups

Keywords

  • Endothelial vesicles
  • Extremity vessels
  • Gravitational physiology
  • Head-up tilt
  • Orthostatic tolerance

ASJC Scopus subject areas

  • Physiology

Cite this

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title = "Selective suppression of an endothelin and platelet-derived growth factor containing vesicular system in endothelium of rat saphenous vein by long-term orthostasis",
abstract = "Electron-dense vesicles were observed in rat vascular endothelium. The purpose of this study was to characterize their content(s), venous-arterial distribution and response to chronic orthostatic stress in extremity vessels. Saphenous and brachial vessels - saphenous vein (SV), saphenous artery (SA), brachial vein, brachial artery - were prepared for electron microscopy to quantitate the vesicle area within the endothelium following immunohistochemical and immunocytochemical identification. The effect of long-term orthostasis was assessed by exposure to head-up tilt for 2 weeks. The vesicular area in relation to the total cross-sectional area of the endothelial cells in the SV and SA of normal and confined control groups was 3.88 ± 0.38 versus 0.89 ± 0.06{\%} (p <0.05) and 4.92 ± 0.25 versus 1.09 ± 0.47{\%} (p <0.05), respectively. Head-up tilt suppressed the vesicle content of the SV to 2.26 ± 0.39{\%} (p <0.05), but it remained low in the SA (1.29 ± 0.45{\%}), brachial vein (0.45 ± 0.12{\%}) and brachial artery (0.59 ± 0.17{\%}). Endothelin and platelet-derived growth factor, but not acidic phosphatase activity or lipid content, could be identified in the vesicles. Plasma endothelin levels were unchanged. We conclude that dense vesicles in the endothelium of extremity vessels are not cell degradation products. They may represent a vesicular secretory or storage system for endothelin and platelet-derived growth factor which participates in regional vascular adaptation to long-term orthostatic load.",
keywords = "Endothelial vesicles, Extremity vessels, Gravitational physiology, Head-up tilt, Orthostatic tolerance",
author = "G{\'a}bor Raffai and E. Feh{\'e}r and G. N{\'a}dasy and S. Paku and G{\'a}bor Pog{\'a}ny and Ferenc T{\'i}m{\'a}r and B. Szende and E. Monos",
year = "2005",
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doi = "10.1159/000084404",
language = "English",
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TY - JOUR

T1 - Selective suppression of an endothelin and platelet-derived growth factor containing vesicular system in endothelium of rat saphenous vein by long-term orthostasis

AU - Raffai, Gábor

AU - Fehér, E.

AU - Nádasy, G.

AU - Paku, S.

AU - Pogány, Gábor

AU - Tímár, Ferenc

AU - Szende, B.

AU - Monos, E.

PY - 2005/3

Y1 - 2005/3

N2 - Electron-dense vesicles were observed in rat vascular endothelium. The purpose of this study was to characterize their content(s), venous-arterial distribution and response to chronic orthostatic stress in extremity vessels. Saphenous and brachial vessels - saphenous vein (SV), saphenous artery (SA), brachial vein, brachial artery - were prepared for electron microscopy to quantitate the vesicle area within the endothelium following immunohistochemical and immunocytochemical identification. The effect of long-term orthostasis was assessed by exposure to head-up tilt for 2 weeks. The vesicular area in relation to the total cross-sectional area of the endothelial cells in the SV and SA of normal and confined control groups was 3.88 ± 0.38 versus 0.89 ± 0.06% (p <0.05) and 4.92 ± 0.25 versus 1.09 ± 0.47% (p <0.05), respectively. Head-up tilt suppressed the vesicle content of the SV to 2.26 ± 0.39% (p <0.05), but it remained low in the SA (1.29 ± 0.45%), brachial vein (0.45 ± 0.12%) and brachial artery (0.59 ± 0.17%). Endothelin and platelet-derived growth factor, but not acidic phosphatase activity or lipid content, could be identified in the vesicles. Plasma endothelin levels were unchanged. We conclude that dense vesicles in the endothelium of extremity vessels are not cell degradation products. They may represent a vesicular secretory or storage system for endothelin and platelet-derived growth factor which participates in regional vascular adaptation to long-term orthostatic load.

AB - Electron-dense vesicles were observed in rat vascular endothelium. The purpose of this study was to characterize their content(s), venous-arterial distribution and response to chronic orthostatic stress in extremity vessels. Saphenous and brachial vessels - saphenous vein (SV), saphenous artery (SA), brachial vein, brachial artery - were prepared for electron microscopy to quantitate the vesicle area within the endothelium following immunohistochemical and immunocytochemical identification. The effect of long-term orthostasis was assessed by exposure to head-up tilt for 2 weeks. The vesicular area in relation to the total cross-sectional area of the endothelial cells in the SV and SA of normal and confined control groups was 3.88 ± 0.38 versus 0.89 ± 0.06% (p <0.05) and 4.92 ± 0.25 versus 1.09 ± 0.47% (p <0.05), respectively. Head-up tilt suppressed the vesicle content of the SV to 2.26 ± 0.39% (p <0.05), but it remained low in the SA (1.29 ± 0.45%), brachial vein (0.45 ± 0.12%) and brachial artery (0.59 ± 0.17%). Endothelin and platelet-derived growth factor, but not acidic phosphatase activity or lipid content, could be identified in the vesicles. Plasma endothelin levels were unchanged. We conclude that dense vesicles in the endothelium of extremity vessels are not cell degradation products. They may represent a vesicular secretory or storage system for endothelin and platelet-derived growth factor which participates in regional vascular adaptation to long-term orthostatic load.

KW - Endothelial vesicles

KW - Extremity vessels

KW - Gravitational physiology

KW - Head-up tilt

KW - Orthostatic tolerance

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U2 - 10.1159/000084404

DO - 10.1159/000084404

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