Selective inhibition of potassium-stimulated rat adrenal glomerulosa cells by ruthenium red

György Szabadkai, Péter Várnai, Péter Enyedi

Research output: Contribution to journalArticle

10 Citations (Scopus)


The effect of the cationic dye, ruthenium red (RR), on ionic fluxes, Ca2+ signal generation, and stimulation of aldosterone production was studied in isolated rat adrenal glomerulosa cells. In these cells, increased extracellular [K+] as well as angiotensin II (Ang II) elevate cytoplasmic Ca2+ concentration and thereupon activate steroidogenesis. However, the mode of action of the two stimuli are different: while a dihidropyridine-sensitive mechanism contributes to the response to both agonists, Ang II induces Ca2+ release from intracellular stores and causes capacitative Ca2+ influx, whereas K+ was recently shown to activate a plasma membrane Ca2+ current (I(gl)) independently of membrane depolarization. The difference is reflected in the sensitivity of the response of the cells to RR. The Ang II-induced Ca2+ signal and aldosterone production were not inhibited, but rather slightly potentiated by the dye. This potentiation was probably the consequence of the membrane-depolarizing effect of RR, due to the observed inhibition of the resting K+ conductance. Conversely, Ca2+ signal and aldosterone production were significantly reduced by RR when the cells were stimulated by moderately elevated [K+] (6-8 mM). Our patch clamp studies suggest that this effect was related to the inhibition of different voltage-dependent and -independent inward Ca2+ currents and indicates the functional importance of the latter in the signal transduction of the potassium-stimulated glomerulosa cell. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalBiochemical Pharmacology
Issue number2
Publication statusPublished - Jan 15 1999


  • Aldosterone
  • I(gl)
  • Rat adrenal glomerulosa cell
  • Ruthenium red
  • Voltage-dependent Ca channel

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Fingerprint Dive into the research topics of 'Selective inhibition of potassium-stimulated rat adrenal glomerulosa cells by ruthenium red'. Together they form a unique fingerprint.

  • Cite this