Selective inhibition of chlorophyll biosynthesis by nicotinamide

Yuzo Shioi, Michio Doi, B. Böddi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Rhodobacter sphaeroides grown in the presence of nicotinamide excreted bacteriochlorophyll precursors, 2,4-divinyl protochlorophyllide (DV-Pchlide) and a small amount of 2-monovinyl protochlorophyllide (MV-Pchlide). Accumulation of these pigments indicates that nicotinamide inhibited the bacteriochlorophyll biosynthetic pathway site-specifically between DV-Pchlide and MV-Pchlide. This phenomenon is also observed in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114. Among 12 nicotinamide derivatives and isomers tested, only nicotinamide was effective, indicating that in addition to the completeness of the pyridine ring skeleton at positions 1 to 3, the carboxylic acid amide group is essential for this inhibition. The technique described in this report permits the simple preparation of large quantities of DV-Pchlide.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume267
Issue number1
DOIs
Publication statusPublished - Nov 15 1988

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Niacinamide
Biosynthesis
Chlorophyll
Protochlorophyllide
Bacteriochlorophylls
Sphingomonadaceae
Rhodobacter sphaeroides
Aerobic Bacteria
Biosynthetic Pathways
Carboxylic Acids
Skeleton
Pigments
Amides
Isomers
Bacteria
Derivatives
2,4-divinylprotochlorophyllide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Selective inhibition of chlorophyll biosynthesis by nicotinamide. / Shioi, Yuzo; Doi, Michio; Böddi, B.

In: Archives of Biochemistry and Biophysics, Vol. 267, No. 1, 15.11.1988, p. 69-74.

Research output: Contribution to journalArticle

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