Selective conversion of fibroblasts into peripheral sensory neurons

Joel W. Blanchard, Kevin T. Eade, Attila Szůcs, Valentina Lo Sardo, Rachel K. Tsunemoto, Daniel Williams, Pietro Paolo Sanna, Kristin K. Baldwin

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73 Citations (Scopus)


Humans and mice detect pain, itch, temperature, pressure, stretch and limb position via signaling from peripheral sensory neurons. These neurons are divided into three functional classes (nociceptors/pruritoceptors, mechanoreceptors and proprioceptors) that are distinguished by their selective expression of TrkA, TrkB or TrkC receptors, respectively. We found that transiently coexpressing Brn3a with either Ngn1 or Ngn2 selectively reprogrammed human and mouse fibroblasts to acquire key properties of these three classes of sensory neurons. These induced sensory neurons (iSNs) were electrically active, exhibited distinct sensory neuron morphologies and matched the characteristic gene expression patterns of endogenous sensory neurons, including selective expression of Trk receptors. In addition, we found that calcium-imaging assays could identify subsets of iSNs that selectively responded to diverse ligands known to activate itch-and pain-sensing neurons. These results offer a simple and rapid means for producing genetically diverse human sensory neurons suitable for drug screening and mechanistic studies.

Original languageEnglish
Pages (from-to)25-35
Number of pages11
JournalNature Neuroscience
Issue number1
Publication statusPublished - Jan 1 2015


ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Blanchard, J. W., Eade, K. T., Szůcs, A., Lo Sardo, V., Tsunemoto, R. K., Williams, D., Sanna, P. P., & Baldwin, K. K. (2015). Selective conversion of fibroblasts into peripheral sensory neurons. Nature Neuroscience, 18(1), 25-35.