Selective artificial receptors based on micropatterned surface-imprinted polymers for label-free detection of proteins by SPR imaging

Gergely Lautner, Jevgeni Kaev, Jekaterina Reut, Andres Öpik, Jörg Rappich, Vitali Syritski, Róbert E. Gyurcsányi

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A novel concept to generate micropatterned surface-imprinted polymers (SIPs) for protein recognition by using standard photolithographic technology is introduced. Avidin-imprinted poly(3,4-ethylenedioxythiophene)/ poly(styrenesulfonate) (PEDOT/PSS) conducting polymer microbands are prepared directly on surface plasmon resonance (SPR) chips, which enable convenient label-free monitoring of the binding events. The novel surface-imprinted microstructures bind avidin, the template protein, with dissociation constants in the submicromolar range (125 nM). The SIPs have an avidin binding capacity approximately one order of magnitude higher than the corresponding nonimprinted polymers and are able to discriminate among functional homologues of avidin, i.e., neutravidin, extravidin, and streptavidin. A novel concept to generate micropatterned surface-imprinted polymers for protein recognition by photolithography is introduced. The avidin-imprinted poly(3,4- ethylenedioxythiophene) (PEDOT) doped with poly(styrenesulfonate) (PSS) bands prepared directly on surface plasmon resonance (SPR) imaging chips enable the label-free monitoring of the binding events. The surface-imprinted microstructures discriminate among functional homologues of avidin.

Original languageEnglish
Pages (from-to)591-597
Number of pages7
JournalAdvanced Functional Materials
Volume21
Issue number3
DOIs
Publication statusPublished - Feb 8 2011

Keywords

  • molecularly imprinted polymers
  • photolithographic micropatterning
  • protein recognition
  • surface imprinting
  • surface plasmon resonance imaging

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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