Selection for Run1-Ren1 dihybrid grapevines using microsatellite markers

Chen Li, Alexandra Erwin, Dániel Pap, Courtney Coleman, Alyssa D. Higgins, E. Kiss, Pál Kozma, Sarolta Hoffmann, David W. Ramming, László G. Kovács

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A grapevine hybrid progeny was generated to track the inheritance of the Ren1 and the Run1 powdery mildew resistance alleles and the segregation of the powdery mildew resistance phenotype. Genotypic analysis was carried out using flanking microsatellite markers; phenotypic evaluations were done under in vitro and greenhouse conditions. Pairing the phenotypic and genotypic data demonstrated that Ren1 and Run1 acted as single dominant loci and assorted independently without considerable distortion of segregation. Chromosomal recombination events were detected in the Ren1 but not in the Run1 region, corroborating earlier observations that crossover between homologous chromosomes was suppressed around the Run1 locus. Taken together, the results confirmed that microsatellite marker-assisted selection is a reliable and expeditious method to combine multiple alleles that confer resistance to a pathogen.

Original languageEnglish
Pages (from-to)152-155
Number of pages4
JournalAmerican Journal of Enology and Viticulture
Volume64
Issue number1
DOIs
Publication statusPublished - Mar 2013

Fingerprint

powdery mildew
Microsatellite Repeats
Alleles
microsatellite repeats
alleles
segregation distortion
loci
marker-assisted selection
Genetic Recombination
inheritance (genetics)
Chromosomes
chromosomes
greenhouses
Phenotype
phenotype
pathogens
methodology
In Vitro Techniques

Keywords

  • Erysiphe necator
  • Gene pyramiding
  • Marker-assisted selection
  • Microsatellite
  • Ren1
  • Run1

ASJC Scopus subject areas

  • Food Science
  • Horticulture

Cite this

Selection for Run1-Ren1 dihybrid grapevines using microsatellite markers. / Li, Chen; Erwin, Alexandra; Pap, Dániel; Coleman, Courtney; Higgins, Alyssa D.; Kiss, E.; Kozma, Pál; Hoffmann, Sarolta; Ramming, David W.; Kovács, László G.

In: American Journal of Enology and Viticulture, Vol. 64, No. 1, 03.2013, p. 152-155.

Research output: Contribution to journalArticle

Li, C, Erwin, A, Pap, D, Coleman, C, Higgins, AD, Kiss, E, Kozma, P, Hoffmann, S, Ramming, DW & Kovács, LG 2013, 'Selection for Run1-Ren1 dihybrid grapevines using microsatellite markers', American Journal of Enology and Viticulture, vol. 64, no. 1, pp. 152-155. https://doi.org/10.5344/ajev.2012.12060
Li, Chen ; Erwin, Alexandra ; Pap, Dániel ; Coleman, Courtney ; Higgins, Alyssa D. ; Kiss, E. ; Kozma, Pál ; Hoffmann, Sarolta ; Ramming, David W. ; Kovács, László G. / Selection for Run1-Ren1 dihybrid grapevines using microsatellite markers. In: American Journal of Enology and Viticulture. 2013 ; Vol. 64, No. 1. pp. 152-155.
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