Selecting a suitable LC column for pharmaceutical separations using a column characterisation system

Erik Haghedooren, Tom Janssens, Ruben Nijs, Soo Kyung Park, Edit Farkas, Sanja Dragovic, Béla Noszál, Jos Hoogmartens, Erwin Adams

Research output: Contribution to journalArticle

4 Citations (Scopus)


Selecting a reversed phase liquid chromatographic (RP-LC) column with suitable selectivity for a particular separation is difficult if the brand name of the column is not known. Monographs of the European Pharmacopoeia and other official compendia for drug analysis only give a general description of the stationary phase to be used for a liquid chromatographic method. A project to develop a chromatographic test procedure to characterise RP-LC C18 columns was started earlier and resulted in a fast, simple, repeatable, and reproducible test procedure. Four column parameters allowed the characterisation and ranking of these columns. In this paper, the separations of three drug substances (amlodipine, tetracaine, and bisacodyl) from their respective impurities were examined applied on 77 RP-LC columns. It was observed, that the column ranking system was helpful in the selection of a suitable column for the separation of amlodipineand tetracaine, but also showed its limitations towards the separation of bisacodyl.

Original languageEnglish
Pages (from-to)747-771
Number of pages25
JournalJournal of Liquid Chromatography and Related Technologies
Issue number6
Publication statusPublished - Jan 1 2009


  • Amlodipine
  • Bisacodyl
  • Chromatographic tests
  • Column ranking
  • Tetracaine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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