Selected papers from the Fourth Annual Meeting on Mechanisms in Local Immunity and Fourth Meeting of the Alps-Adria Society for Immunology of Reproduction, Opatija, Croatia, 16-19 September 1998: Progesterone-induced blocking factor inhibits degranulation of natural killer cells

Zs Faust, G. Laškarin, D. Rukavina, J. Szekeres-Barthó

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108 Citations (Scopus)

Abstract

PROBLEM: During the first trimester of pregnancy, nonclassical (CD3-, CD56+, CD16-, perforin [P](bright+)) natural killer (NK) cells comprise the major decidual lymphocyte population. These cells, in spite of their high perforin content, exert a low cytolytic activity. Peripheral blood lymphocytes of healthy pregnant women produce progesterone-induced blocking factor (PIBF), which inhibits NK activity. PIBF-producing cells are likely to be present in decidua and might contribute to low decidual NK activity. METHOD OF STUDY: Decidual cells obtained from elective pregnancy termination were double labeled for CD56 and PIBF. We tested the effect of PIBF on perforin liberation by activated peripheral blood NK cells. RESULTS: Sixty percent of decidual lymphocytes were CD56+ and expressed PIBF at the same time. PIBF-treated and untreated peripheral blood NK cells were incubated with K-562 cells, and perforin content of target conjugated NK cells was detected with immunocytochemistry. PIBF treatment of peripheral blood lymphocytes significantly reduced lysis of K-562 cells. Among target bound lymphocytes in PIBF-treated samples, we found a significantly (P <0.01) higher rate of P+ cells than in untreated samples. CONCLUSIONS: These data suggest that PIBF inhibits cytotoxicity of NK cells via a block of degranulation, and since decidual NK cells are PIBF+, it cannot be ruledout that this effect of PIBF contributes to low decidual NK activity.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalAmerican Journal of Reproductive Immunology
Volume42
Issue number2
Publication statusPublished - 1999

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Croatia
Allergy and Immunology
Natural Killer Cells
Reproduction
Progesterone
Immunity
Perforin
Lymphocytes
Blood Cells
blocking factor
Decidua
First Pregnancy Trimester
Pregnant Women
Immunohistochemistry

Keywords

  • Decidua
  • Degranulation
  • NK cells
  • Perforin
  • PIBF

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynaecology

Cite this

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title = "Selected papers from the Fourth Annual Meeting on Mechanisms in Local Immunity and Fourth Meeting of the Alps-Adria Society for Immunology of Reproduction, Opatija, Croatia, 16-19 September 1998: Progesterone-induced blocking factor inhibits degranulation of natural killer cells",
abstract = "PROBLEM: During the first trimester of pregnancy, nonclassical (CD3-, CD56+, CD16-, perforin [P](bright+)) natural killer (NK) cells comprise the major decidual lymphocyte population. These cells, in spite of their high perforin content, exert a low cytolytic activity. Peripheral blood lymphocytes of healthy pregnant women produce progesterone-induced blocking factor (PIBF), which inhibits NK activity. PIBF-producing cells are likely to be present in decidua and might contribute to low decidual NK activity. METHOD OF STUDY: Decidual cells obtained from elective pregnancy termination were double labeled for CD56 and PIBF. We tested the effect of PIBF on perforin liberation by activated peripheral blood NK cells. RESULTS: Sixty percent of decidual lymphocytes were CD56+ and expressed PIBF at the same time. PIBF-treated and untreated peripheral blood NK cells were incubated with K-562 cells, and perforin content of target conjugated NK cells was detected with immunocytochemistry. PIBF treatment of peripheral blood lymphocytes significantly reduced lysis of K-562 cells. Among target bound lymphocytes in PIBF-treated samples, we found a significantly (P <0.01) higher rate of P+ cells than in untreated samples. CONCLUSIONS: These data suggest that PIBF inhibits cytotoxicity of NK cells via a block of degranulation, and since decidual NK cells are PIBF+, it cannot be ruledout that this effect of PIBF contributes to low decidual NK activity.",
keywords = "Decidua, Degranulation, NK cells, Perforin, PIBF",
author = "Zs Faust and G. Laškarin and D. Rukavina and J. Szekeres-Barth{\'o}",
year = "1999",
language = "English",
volume = "42",
pages = "71--75",
journal = "American Journal of Reproductive Immunology",
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T1 - Selected papers from the Fourth Annual Meeting on Mechanisms in Local Immunity and Fourth Meeting of the Alps-Adria Society for Immunology of Reproduction, Opatija, Croatia, 16-19 September 1998

T2 - Progesterone-induced blocking factor inhibits degranulation of natural killer cells

AU - Faust, Zs

AU - Laškarin, G.

AU - Rukavina, D.

AU - Szekeres-Barthó, J.

PY - 1999

Y1 - 1999

N2 - PROBLEM: During the first trimester of pregnancy, nonclassical (CD3-, CD56+, CD16-, perforin [P](bright+)) natural killer (NK) cells comprise the major decidual lymphocyte population. These cells, in spite of their high perforin content, exert a low cytolytic activity. Peripheral blood lymphocytes of healthy pregnant women produce progesterone-induced blocking factor (PIBF), which inhibits NK activity. PIBF-producing cells are likely to be present in decidua and might contribute to low decidual NK activity. METHOD OF STUDY: Decidual cells obtained from elective pregnancy termination were double labeled for CD56 and PIBF. We tested the effect of PIBF on perforin liberation by activated peripheral blood NK cells. RESULTS: Sixty percent of decidual lymphocytes were CD56+ and expressed PIBF at the same time. PIBF-treated and untreated peripheral blood NK cells were incubated with K-562 cells, and perforin content of target conjugated NK cells was detected with immunocytochemistry. PIBF treatment of peripheral blood lymphocytes significantly reduced lysis of K-562 cells. Among target bound lymphocytes in PIBF-treated samples, we found a significantly (P <0.01) higher rate of P+ cells than in untreated samples. CONCLUSIONS: These data suggest that PIBF inhibits cytotoxicity of NK cells via a block of degranulation, and since decidual NK cells are PIBF+, it cannot be ruledout that this effect of PIBF contributes to low decidual NK activity.

AB - PROBLEM: During the first trimester of pregnancy, nonclassical (CD3-, CD56+, CD16-, perforin [P](bright+)) natural killer (NK) cells comprise the major decidual lymphocyte population. These cells, in spite of their high perforin content, exert a low cytolytic activity. Peripheral blood lymphocytes of healthy pregnant women produce progesterone-induced blocking factor (PIBF), which inhibits NK activity. PIBF-producing cells are likely to be present in decidua and might contribute to low decidual NK activity. METHOD OF STUDY: Decidual cells obtained from elective pregnancy termination were double labeled for CD56 and PIBF. We tested the effect of PIBF on perforin liberation by activated peripheral blood NK cells. RESULTS: Sixty percent of decidual lymphocytes were CD56+ and expressed PIBF at the same time. PIBF-treated and untreated peripheral blood NK cells were incubated with K-562 cells, and perforin content of target conjugated NK cells was detected with immunocytochemistry. PIBF treatment of peripheral blood lymphocytes significantly reduced lysis of K-562 cells. Among target bound lymphocytes in PIBF-treated samples, we found a significantly (P <0.01) higher rate of P+ cells than in untreated samples. CONCLUSIONS: These data suggest that PIBF inhibits cytotoxicity of NK cells via a block of degranulation, and since decidual NK cells are PIBF+, it cannot be ruledout that this effect of PIBF contributes to low decidual NK activity.

KW - Decidua

KW - Degranulation

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