Secretion of phospholipase A2 induced by interactions of human platelets with monocytes

S. Sipka, T. Farkas, P. Gergely, L. Bali, J. Laczko, S. Szabados, I. Csípő, M. Koltai, G. Szegedi

Research output: Contribution to journalArticle

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Abstract

Phospholipase A2 (PLA2) activity was found and measured in the cell-free supernatants of human mononuclear cells (monocytes and lymphocytes) cultured with platelets for 48 h at 37° C. The relative molecular mass of purified, calcium-dependent PLA2 was 14 kD. The amount of PLA2 in the supernatants correlated positively with the number of monocytes and platelets in the cultures. Electron microscopically, direct cell-to-cell interactions of monocytes and platelets were observed. Cultivation of suspensions of human mononuclear cells with platelets in serum-free medium was found to be an efficient way to produce and purify human secretable PLA2. In the release of secretable PLA2 in peripheral blood, the interactions between platelets and monocytes may play a considerable role.

Original languageEnglish
Pages (from-to)307-310
Number of pages4
JournalAnnals of Hematology
Volume69
Issue number6
DOIs
Publication statusPublished - Dec 1994

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Phospholipases A2
Monocytes
Blood Platelets
Serum-Free Culture Media
Platelet Count
Cell Communication
Suspensions
Lymphocytes
Electrons
Calcium

Keywords

  • Monocyte
  • Phospholipase A
  • Platelet

ASJC Scopus subject areas

  • Hematology

Cite this

Secretion of phospholipase A2 induced by interactions of human platelets with monocytes. / Sipka, S.; Farkas, T.; Gergely, P.; Bali, L.; Laczko, J.; Szabados, S.; Csípő, I.; Koltai, M.; Szegedi, G.

In: Annals of Hematology, Vol. 69, No. 6, 12.1994, p. 307-310.

Research output: Contribution to journalArticle

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AU - Gergely, P.

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AU - Szabados, S.

AU - Csípő, I.

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