Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits

Andrea Kerekes, Orsolya Ivett Hoffmann, Gergely Iski, Nándor Lipták, Elen Gócza, Wilfried A. Kues, Z. Bösze, László Hiripi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Transgenic rabbits carrying mammary gland specific gene constructs are extensively used for excreting recombinant proteins into the milk. Here, we report refined phenotyping of previously generated Venus transposon-carrying transgenic rabbits with particular emphasis on the secretion of the reporter protein by exocrine glands, such as mammary, salivary, tear and seminal glands. The Sleeping Beauty (SB) transposon transgenic construct contains the Venus fluorophore cDNA, but without a signal peptide for the secretory pathway, driven by the ubiquitous CAGGS (CAG) promoter. Despite the absence of a signal peptide, the fluorophore protein was readily detected in milk, tear, saliva and seminal fluids. The expression pattern was verified by Western blot analysis. Mammary gland epithelial cells of SB-CAG-Venus transgenic lactating does also showed Venus-specific expression by tissue histology and fluorescence microscopy. In summary, the SB-CAG-Venus transgenic rabbits secrete the recombinant protein by different glands. This finding has relevance not only for the understanding of the biological function of exocrine glands, but also for the design of constructs for expression of recombinant proteins in dairy animals.

Original languageEnglish
Article numbere0187214
JournalPLoS One
Volume12
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Venus
Exocrine Glands
exocrine glands
Protein Sorting Signals
signal peptide
Recombinant Proteins
recombinant proteins
Fluorophores
Beauty
rabbits
secretion
genetically modified organisms
Rabbits
fluorescent dyes
Human Mammary Glands
Histology
Tears
Dairies
mammary glands
transposons

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Kerekes, A., Hoffmann, O. I., Iski, G., Lipták, N., Gócza, E., Kues, W. A., ... Hiripi, L. (2017). Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits. PLoS One, 12(10), [e0187214]. https://doi.org/10.1371/journal.pone.0187214

Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits. / Kerekes, Andrea; Hoffmann, Orsolya Ivett; Iski, Gergely; Lipták, Nándor; Gócza, Elen; Kues, Wilfried A.; Bösze, Z.; Hiripi, László.

In: PLoS One, Vol. 12, No. 10, e0187214, 01.10.2017.

Research output: Contribution to journalArticle

Kerekes, A, Hoffmann, OI, Iski, G, Lipták, N, Gócza, E, Kues, WA, Bösze, Z & Hiripi, L 2017, 'Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits', PLoS One, vol. 12, no. 10, e0187214. https://doi.org/10.1371/journal.pone.0187214
Kerekes, Andrea ; Hoffmann, Orsolya Ivett ; Iski, Gergely ; Lipták, Nándor ; Gócza, Elen ; Kues, Wilfried A. ; Bösze, Z. ; Hiripi, László. / Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits. In: PLoS One. 2017 ; Vol. 12, No. 10.
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