Secretion from minor salivary glands following ablation of the major salivary glands in rats

J. Blazsek, G. Varga

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Since minor salivary glands are tiny and dispersed, ductal cannulation cannot be used when studying their function. The present study was devised to develop a method of measuring minor salivary gland function by excision of the major glands. Female rats (230-280 g) were anaesthetized with sodium pentobarbital. Ablation of the submandibular, sublingual and parotid glands was performed through a sagittal neck incision. Sham-operated rats served as controls. Groups of sialadenectomized animals were investigated immediately and after 1 week, 2 weeks and 3 months. To study secretory function, the mouth was rinsed with 250 μl water in every 5 min and protein and amylase concentrations were measured. After an initial 50 min of basal secretion pilocarpine (1 mg/kg, i.p.) was given. Bilateral ablation of both submandibular, sublingual and parotid glands led to a moderate loss of body weight and a considerable increase in water intake. No other obvious abnormality was observed for periods up to 90 days following surgery. We deduce that the minor glands secrete approximately 14% of protein and 1% of amylase in whole saliva. Secretion is maintained even after 90 days following removal of the major glands. Surgical removal of the major salivary glands allows the secretory function of the minor glands in rats to be studied in vivo.

Original languageEnglish
JournalArchives of Oral Biology
Volume44
Issue numberSUPPL. 1
DOIs
Publication statusPublished - May 1999

Fingerprint

Minor Salivary Glands
Salivary Glands
Sublingual Gland
Submandibular Gland
Parotid Gland
Amylases
Pilocarpine
Pentobarbital
Ambulatory Surgical Procedures
Saliva
Catheterization
Drinking
Mouth
Proteins
Neck
Body Weight
Water

Keywords

  • Ablation
  • Amylase
  • Minor salivary glands
  • Protein
  • Rat
  • Secretion

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Secretion from minor salivary glands following ablation of the major salivary glands in rats. / Blazsek, J.; Varga, G.

In: Archives of Oral Biology, Vol. 44, No. SUPPL. 1, 05.1999.

Research output: Contribution to journalArticle

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