Screening stress tolerance traits in Arabidopsis cell cultures

Imma Pérez-Salamó, Bogáta Boros, L. Szabados

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Screening for tolerance traits in plant cell cultures can combine the efficiency of microbial selection and plant genetics. Agrobacterium-mediated transformation can efficiently introduce cDNA library to cell suspension cultures generating population of randomly transformed microcolonies. Transformed cultures can subsequently be screened for tolerance to different stress conditions such as salinity, high osmotic, or oxidative stress conditions. cDNA inserts in tolerant cell lines can be easily identified by PCR amplification and homology search of the determined nucleotide sequences. The described methods have been tested and used to identify regulatory genes controlling salt tolerance in Arabidopsis. As cDNA libraries can be prepared from any plants, natural diversity can be explored by using extremophile plants as gene source.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages235-246
Number of pages12
Volume1398
DOIs
Publication statusPublished - Feb 1 2016

Publication series

NameMethods in Molecular Biology
Volume1398
ISSN (Print)10643745

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Keywords

  • Agrobacterium
  • CDNA library
  • Gene isolation
  • In vitro selection
  • Transformation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Pérez-Salamó, I., Boros, B., & Szabados, L. (2016). Screening stress tolerance traits in Arabidopsis cell cultures. In Methods in Molecular Biology (Vol. 1398, pp. 235-246). (Methods in Molecular Biology; Vol. 1398). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3356-3_19