Screening of Hungarian cattle herds for Mycoplasma mycoides subspecies mycoides small colony infection with negative results

L. Stipkovits, A. Dán, Erika Varga, Paula De Santis, Rosella Lelly, Éva Kaszanyitzky, Ildikó Ferenczné Paluska, M. Tenk, L. Tekes, B. Harrach

Research output: Contribution to journalArticle

Abstract

At abattoirs and farms, 1248 sera were collected from animals representing 121 farms, and examined by complement fixation test using Mycoplasma mycoides subspecies mycoides small colony type (MmmSC) antigen. All sera were negative except seven from four farms, giving ++ reactions in the serum dilution of 1:10. On retesting, these sera and additional 30 sera collected repeatedly in both farms gave negative results. In isolation attempts, 953 lung samples collected from slaughtered cattle at the same abattoirs, and 326 nasal swabs collected from 11 herds proved to be negative for the presence of MmmSC, but M. bovis was isolated frequently. In the small farms 23.95% of the animals had pleurisy and/or pneumonia while in the large herds 34.69% had lesions. DNA extracted from 50 nasal swabs and 430 lung samples was examined by polymerase chain reaction (PCR) using M. mycoides cluster-specific primers. DNA from further 325 lung samples was tested by the more specific M. mycoides subspecies mycoides small colony/large colony/capri specific primers and 196 samples by nested PCR specific for MmmSC. All gave negative results. The detection level of cluster-specific primers and the more specific primers was 33.4 pg of DNA, whereas that of nested PCR was 0.33 pg.

Original languageEnglish
Pages (from-to)375-385
Number of pages11
JournalActa Veterinaria Hungarica
Volume48
Issue number4
Publication statusPublished - 2000

Fingerprint

Mycoplasma mycoides
herds
screening
cattle
Infection
Serum
farms
Abattoirs
infection
polymerase chain reaction
lungs
Nose
Polymerase Chain Reaction
slaughterhouses
Lung
DNA
Complement Fixation Tests
sampling
Pleurisy
complement fixation tests

Keywords

  • Complement fixation test
  • Contagious bovine pleuropneumonia
  • Culturing of Mycoplasma mycoides subsp. mycoides SC
  • M. bovis
  • PCR

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Screening of Hungarian cattle herds for Mycoplasma mycoides subspecies mycoides small colony infection with negative results. / Stipkovits, L.; Dán, A.; Varga, Erika; De Santis, Paula; Lelly, Rosella; Kaszanyitzky, Éva; Ferenczné Paluska, Ildikó; Tenk, M.; Tekes, L.; Harrach, B.

In: Acta Veterinaria Hungarica, Vol. 48, No. 4, 2000, p. 375-385.

Research output: Contribution to journalArticle

Stipkovits, L, Dán, A, Varga, E, De Santis, P, Lelly, R, Kaszanyitzky, É, Ferenczné Paluska, I, Tenk, M, Tekes, L & Harrach, B 2000, 'Screening of Hungarian cattle herds for Mycoplasma mycoides subspecies mycoides small colony infection with negative results', Acta Veterinaria Hungarica, vol. 48, no. 4, pp. 375-385.
Stipkovits, L. ; Dán, A. ; Varga, Erika ; De Santis, Paula ; Lelly, Rosella ; Kaszanyitzky, Éva ; Ferenczné Paluska, Ildikó ; Tenk, M. ; Tekes, L. ; Harrach, B. / Screening of Hungarian cattle herds for Mycoplasma mycoides subspecies mycoides small colony infection with negative results. In: Acta Veterinaria Hungarica. 2000 ; Vol. 48, No. 4. pp. 375-385.
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