Screening of different Kluyveromyces strains for simultaneous saccharification and fermentation

M. Bollók, K. Réczey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

It is of big importance to find a thermotolerant yeast strain with a high tolerance against inhibitors present in steam-pretreated wood, which can be used in simultaneous saccharification and fermentation (SSF) process for bioethanol production from wood. Five thermotolerant yeast strains belonging to the genera Kluyveromyces marxianus (3) and Kluyveromyces thermotolerans (2) were studied by growing them on agar slants and in shake flasks at different temperatures using glucose medium. The yeasts were grown in the presence of the liquid of steam-pretreated softwood as well to test the tolerance of yeasts against inhibitors formed during the pretreatment of wood. The investigated yeasts proved to be sensitive to inhibitors at high temperatures. K. marxianus Y.00243 showed less sensitivity to inhibitors at 37 °C, K. marxianus Y.01070 was the most thermotolerant on glucose medium, but it was too sensitive to inhibitors.

Original languageEnglish
Pages (from-to)59-70
Number of pages12
JournalAccreditation and Quality Assurance
Volume5
Issue number1
Publication statusPublished - 2000

Fingerprint

Saccharification
fermentation
yeast
Yeast
Fermentation
inhibitors
Screening
screening
Wood
Steam
glucose
steam
Glucose
flasks
Bioethanol
Softwoods
pretreatment
Agar
slopes
Temperature

Keywords

  • Kluyveromyces marxianus
  • Kluyveromyces thermotolerans
  • Steam-pretreated softwood

ASJC Scopus subject areas

  • Analytical Chemistry
  • Instrumentation

Cite this

Screening of different Kluyveromyces strains for simultaneous saccharification and fermentation. / Bollók, M.; Réczey, K.

In: Accreditation and Quality Assurance, Vol. 5, No. 1, 2000, p. 59-70.

Research output: Contribution to journalArticle

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