Screening for plant regeneration in callus and protoplast cultures of alfalfa (Medicago Sativa L.) germplasms

Su Nam Li, L. Heszky

Research output: Contribution to journalArticle

Abstract

Ovaries, hypocotyls and petioles of 21 germplasm-sources of Medicago saliva L. were used for callus induction and subsequent evaluation of plant regeneration. Alost of the cultivars produced callus as much as 95% over the explants employed. Fifteen cultivars (71%) showed some degrees of plant regeneration. The results support the strong dependence of plant regeneration on genotype, explant and subculture. Cultivars with the highest frequency of regeneration were "Szentesi-délibáh" (46.7%) and "Rambler" (41.7%). The "Szentesi-délibáh" variety having shown a persistence in its regeneration capacity over three subcultures. Protoplasts isolated from Szentesidélibáh and Szentcsi-821 cultivars were cultured in a liquid medium. Plating efficiency was about 40-50%. Healthy plants were regenerated from the protoplasts of these two cultivars.

Original languageEnglish
Pages (from-to)387-393
Number of pages7
JournalActa Botanica Hungarica
Volume33
Issue number3-4
Publication statusPublished - 1987

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germplasm
alfalfa
Medicago sativa
protoplasts
cultivar
callus
regeneration
screening
cultivars
explants
saliva
Medicago
petioles
hypocotyls
genotype
persistence
liquid
liquids

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Screening for plant regeneration in callus and protoplast cultures of alfalfa (Medicago Sativa L.) germplasms. / Li, Su Nam; Heszky, L.

In: Acta Botanica Hungarica, Vol. 33, No. 3-4, 1987, p. 387-393.

Research output: Contribution to journalArticle

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