Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay

G. Nagy, R. Gyurcsányi, Alessandra Cristalli, Michael R. Neuman, Ernö Lindner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A microfabricated amperometric microcell was designed and used for the determination of proline iminopeptidase (PIP) enzyme activity in 2-10-μl samples. The measurements were made in the range of 10.3-841.5 mU/ml enzyme activities. The sensitivity of the determinations was between -0.0195 and -0.0203 μA ml/mU per min. The coefficient of variation of the determined values ranged between 2.8 (at 561.2 mU/ml) and 24.1% (at 10.3 mU/ml). The microcell was manufactured on an alumina substrate using screen-printed graphite working and Ag/AgCl reference electrodes. Elevated PIP activity in the vaginal fluid is a biochemical indicator of bacterial vaginosis. The method is appropriate to differentiate between normal (66±145 mU/ml) and elevated, diseased (704±145 mU/ml), values. Copyright (C) 2000 Elsevier Science S.A.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalBiosensors and Bioelectronics
Volume15
Issue number5-6
DOIs
Publication statusPublished - Aug 2000

Fingerprint

Enzyme Assays
Enzyme activity
Assays
Bacterial Vaginosis
Graphite
Aluminum Oxide
Enzymes
Electrodes
Alumina
Fluids
Substrates
prolyl aminopeptidase

Keywords

  • Enzyme assay
  • Microfabricated amperometric cell
  • Proline iminopeptidase activity
  • Screen-printed graphite electrode

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay. / Nagy, G.; Gyurcsányi, R.; Cristalli, Alessandra; Neuman, Michael R.; Lindner, Ernö.

In: Biosensors and Bioelectronics, Vol. 15, No. 5-6, 08.2000, p. 265-272.

Research output: Contribution to journalArticle

Nagy, G. ; Gyurcsányi, R. ; Cristalli, Alessandra ; Neuman, Michael R. ; Lindner, Ernö. / Screen-printed amperometric microcell for proline iminopeptidase enzyme activity assay. In: Biosensors and Bioelectronics. 2000 ; Vol. 15, No. 5-6. pp. 265-272.
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