Schistosomal egg granuloma-derived fibroblast-stimulating factor is apparently distinct from interleukin-1

S. Prakash, C. A. Dinarello, K. Dankó, M. J. Stadecker, D. J. Wyler

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Abstract

We have previously reported that egg granulomas isolated from livers of Schistosoma mansoni-infected euthymic mice in vitro elaborate a factor(s) that stimulates a variety of fibroblast responses including fibroblast proliferation and enhanced synthesis of extracellular matrix proteins. We have postulated that these factors play a role in the pathogenesis of hepatic fibrosis in schistosomiasis mansoni. Serum-free supernatants from egg granuloma cultures also stimulate thymocyte proliferation in an assay that detects interleukin-1 (IL-1). Thymocytes and fibroblasts are stimulated to proliferate by the same fractions of egg granuloma culture supernatant separated by gel filtration, isoelectric focusing, and ion-exchange chromatography. This suggested that granuloma-derived IL-1 is responsible for the observed fibroblast stimulation. Here we report that the ability of granuloma culture supernatants to stimulate the IL-1-sensitive D10.G4.1 cells but not fibroblasts is removed by treatment with immobilized anti-IL-1 antibody. We also observed that dialyzed culture supernatants from egg granulomas obtained from infected congenitally athymic (nude) mice also stimulate fibroblast proliferation. Treatment with anti-IL-1 antibody did not abrogate this response. In contrast to our experience with egg granulomas isolated from euthymic mice, IL-1- and fibroblast-stimulating activity could be separated by gel filtration and isoelectric focusing. We conclude that the fibroblast growth-stimulating activities elaborated by egg granulomas from S. mansoni-infected euthymic and athymic mice may be different but both appear to be distinct from IL-1.

Original languageEnglish
Pages (from-to)679-684
Number of pages6
JournalInfection and Immunity
Volume57
Issue number3
Publication statusPublished - 1989

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Granuloma
Interleukin-1
Ovum
Fibroblasts
Nude Mice
Schistosomiasis mansoni
Schistosoma mansoni
Isoelectric Focusing
Thymocytes
Gel Chromatography
Antibodies
Extracellular Matrix Proteins
Liver
Ion Exchange Chromatography
Fibrosis
Growth
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Schistosomal egg granuloma-derived fibroblast-stimulating factor is apparently distinct from interleukin-1. / Prakash, S.; Dinarello, C. A.; Dankó, K.; Stadecker, M. J.; Wyler, D. J.

In: Infection and Immunity, Vol. 57, No. 3, 1989, p. 679-684.

Research output: Contribution to journalArticle

Prakash, S, Dinarello, CA, Dankó, K, Stadecker, MJ & Wyler, DJ 1989, 'Schistosomal egg granuloma-derived fibroblast-stimulating factor is apparently distinct from interleukin-1', Infection and Immunity, vol. 57, no. 3, pp. 679-684.
Prakash, S. ; Dinarello, C. A. ; Dankó, K. ; Stadecker, M. J. ; Wyler, D. J. / Schistosomal egg granuloma-derived fibroblast-stimulating factor is apparently distinct from interleukin-1. In: Infection and Immunity. 1989 ; Vol. 57, No. 3. pp. 679-684.
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