Scanning electrochemical microscopy inside the biocatalytic layer of biosensors, investigation of a double function complex multienzyme reaction layer

Baldzs Csóka, Barna Kovács, G. Nagy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As the continuation of the work started earlier, this article deals with the scanning electrochemical microscopic (SECM) study of the function of complex enzyme sensors. In this case reaction layer of the sensor is made of two different parts. One is for interference elimination and the other for selective molecular recognition and measurement. As Scheller's group described this sucrose electrode, the inner that is the measuring layer contains three enzymes: invertase, mutarotase and glucose oxidase while the outer, the eliminating one, uses glucose oxidase and catalase. In our work the concentration profiles of oxygen and hydrogen peroxide were recorded inside the two different enzyme layers with the SECM. The dependence of these profiles on the working parameters such as enzyme activity and activity ratios, reaction layer thickness, sucrose and glucose concentration were observed and compared with the results of model calculations. Method of finite differences was used for the model calculations.

Original languageEnglish
Pages (from-to)1335-1342
Number of pages8
JournalElectroanalysis
Volume15
Issue number15-16
Publication statusPublished - Sep 2003

Fingerprint

Multienzyme Complexes
Glucose Oxidase
Glucose oxidase
Sugar (sucrose)
Biosensors
Sucrose
Microscopic examination
Enzyme sensors
Enzymes
Scanning
beta-Fructofuranosidase
Molecular recognition
Enzyme activity
Hydrogen peroxide
Catalase
Hydrogen Peroxide
Glucose
Oxygen
Electrodes
Sensors

Keywords

  • Biosensor
  • Digital simulation
  • Elimination layer
  • Immobilized enzymes
  • SECM

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Scanning electrochemical microscopy inside the biocatalytic layer of biosensors, investigation of a double function complex multienzyme reaction layer. / Csóka, Baldzs; Kovács, Barna; Nagy, G.

In: Electroanalysis, Vol. 15, No. 15-16, 09.2003, p. 1335-1342.

Research output: Contribution to journalArticle

@article{37303e2c26e0474d95e94365a1a3625a,
title = "Scanning electrochemical microscopy inside the biocatalytic layer of biosensors, investigation of a double function complex multienzyme reaction layer",
abstract = "As the continuation of the work started earlier, this article deals with the scanning electrochemical microscopic (SECM) study of the function of complex enzyme sensors. In this case reaction layer of the sensor is made of two different parts. One is for interference elimination and the other for selective molecular recognition and measurement. As Scheller's group described this sucrose electrode, the inner that is the measuring layer contains three enzymes: invertase, mutarotase and glucose oxidase while the outer, the eliminating one, uses glucose oxidase and catalase. In our work the concentration profiles of oxygen and hydrogen peroxide were recorded inside the two different enzyme layers with the SECM. The dependence of these profiles on the working parameters such as enzyme activity and activity ratios, reaction layer thickness, sucrose and glucose concentration were observed and compared with the results of model calculations. Method of finite differences was used for the model calculations.",
keywords = "Biosensor, Digital simulation, Elimination layer, Immobilized enzymes, SECM",
author = "Baldzs Cs{\'o}ka and Barna Kov{\'a}cs and G. Nagy",
year = "2003",
month = "9",
language = "English",
volume = "15",
pages = "1335--1342",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "15-16",

}

TY - JOUR

T1 - Scanning electrochemical microscopy inside the biocatalytic layer of biosensors, investigation of a double function complex multienzyme reaction layer

AU - Csóka, Baldzs

AU - Kovács, Barna

AU - Nagy, G.

PY - 2003/9

Y1 - 2003/9

N2 - As the continuation of the work started earlier, this article deals with the scanning electrochemical microscopic (SECM) study of the function of complex enzyme sensors. In this case reaction layer of the sensor is made of two different parts. One is for interference elimination and the other for selective molecular recognition and measurement. As Scheller's group described this sucrose electrode, the inner that is the measuring layer contains three enzymes: invertase, mutarotase and glucose oxidase while the outer, the eliminating one, uses glucose oxidase and catalase. In our work the concentration profiles of oxygen and hydrogen peroxide were recorded inside the two different enzyme layers with the SECM. The dependence of these profiles on the working parameters such as enzyme activity and activity ratios, reaction layer thickness, sucrose and glucose concentration were observed and compared with the results of model calculations. Method of finite differences was used for the model calculations.

AB - As the continuation of the work started earlier, this article deals with the scanning electrochemical microscopic (SECM) study of the function of complex enzyme sensors. In this case reaction layer of the sensor is made of two different parts. One is for interference elimination and the other for selective molecular recognition and measurement. As Scheller's group described this sucrose electrode, the inner that is the measuring layer contains three enzymes: invertase, mutarotase and glucose oxidase while the outer, the eliminating one, uses glucose oxidase and catalase. In our work the concentration profiles of oxygen and hydrogen peroxide were recorded inside the two different enzyme layers with the SECM. The dependence of these profiles on the working parameters such as enzyme activity and activity ratios, reaction layer thickness, sucrose and glucose concentration were observed and compared with the results of model calculations. Method of finite differences was used for the model calculations.

KW - Biosensor

KW - Digital simulation

KW - Elimination layer

KW - Immobilized enzymes

KW - SECM

UR - http://www.scopus.com/inward/record.url?scp=0141615969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141615969&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 1335

EP - 1342

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 15-16

ER -