Saturation of membrane lipids by hydrogenation induces thermal stability in chloroplast inhibiting the heat-dependent stimulation of Photosystem I-mediated electron transport

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Abstract

Mild hydrogenation (up to 20%) of cis double bonds of acyl lipids within thylakoid membranes results in a marked increase in the threshold temperature at which heat stress-induced stimulation of DCPIPH2-supported PS I-mediated electron transport is initiated. Lipid saturation above 35% totally prevents the appearance of any increase in electron flow to PS I upon heat pretreatment. Experiments conducted on uncoupled chloroplast show that photosynthetic control of electron transport is not involved in the protective effect associated with lipid saturation. Homogeneous catalytic hydrogenation of unsaturated fatty acids within chloroplast membranes proved to be a powerful technique in verifying that heat stability of chloroplasts is coupled to the level of fatty acid unsaturation.

Original languageEnglish
Pages (from-to)361-364
Number of pages4
JournalBBA - Biomembranes
Volume979
Issue number3
DOIs
Publication statusPublished - Mar 13 1989

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Photosystem I Protein Complex
Hydrogenation
Chloroplasts
Membrane Lipids
Electron Transport
Thermodynamic stability
Hot Temperature
Lipids
Membranes
Thylakoids
Unsaturated Fatty Acids
Fatty Acids
Electrons
Temperature
Experiments

Keywords

  • Catalytic hydrogenation
  • Electron transport
  • Lipid saturation
  • Photosystem I
  • Thermal stability

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

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title = "Saturation of membrane lipids by hydrogenation induces thermal stability in chloroplast inhibiting the heat-dependent stimulation of Photosystem I-mediated electron transport",
abstract = "Mild hydrogenation (up to 20{\%}) of cis double bonds of acyl lipids within thylakoid membranes results in a marked increase in the threshold temperature at which heat stress-induced stimulation of DCPIPH2-supported PS I-mediated electron transport is initiated. Lipid saturation above 35{\%} totally prevents the appearance of any increase in electron flow to PS I upon heat pretreatment. Experiments conducted on uncoupled chloroplast show that photosynthetic control of electron transport is not involved in the protective effect associated with lipid saturation. Homogeneous catalytic hydrogenation of unsaturated fatty acids within chloroplast membranes proved to be a powerful technique in verifying that heat stability of chloroplasts is coupled to the level of fatty acid unsaturation.",
keywords = "Catalytic hydrogenation, Electron transport, Lipid saturation, Photosystem I, Thermal stability",
author = "L. V{\'i}gh and Z. Gombos and I. Horv{\'a}th and Ferenc Jo{\'o}",
year = "1989",
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doi = "10.1016/0005-2736(89)90257-5",
language = "English",
volume = "979",
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T1 - Saturation of membrane lipids by hydrogenation induces thermal stability in chloroplast inhibiting the heat-dependent stimulation of Photosystem I-mediated electron transport

AU - Vígh, L.

AU - Gombos, Z.

AU - Horváth, I.

AU - Joó, Ferenc

PY - 1989/3/13

Y1 - 1989/3/13

N2 - Mild hydrogenation (up to 20%) of cis double bonds of acyl lipids within thylakoid membranes results in a marked increase in the threshold temperature at which heat stress-induced stimulation of DCPIPH2-supported PS I-mediated electron transport is initiated. Lipid saturation above 35% totally prevents the appearance of any increase in electron flow to PS I upon heat pretreatment. Experiments conducted on uncoupled chloroplast show that photosynthetic control of electron transport is not involved in the protective effect associated with lipid saturation. Homogeneous catalytic hydrogenation of unsaturated fatty acids within chloroplast membranes proved to be a powerful technique in verifying that heat stability of chloroplasts is coupled to the level of fatty acid unsaturation.

AB - Mild hydrogenation (up to 20%) of cis double bonds of acyl lipids within thylakoid membranes results in a marked increase in the threshold temperature at which heat stress-induced stimulation of DCPIPH2-supported PS I-mediated electron transport is initiated. Lipid saturation above 35% totally prevents the appearance of any increase in electron flow to PS I upon heat pretreatment. Experiments conducted on uncoupled chloroplast show that photosynthetic control of electron transport is not involved in the protective effect associated with lipid saturation. Homogeneous catalytic hydrogenation of unsaturated fatty acids within chloroplast membranes proved to be a powerful technique in verifying that heat stability of chloroplasts is coupled to the level of fatty acid unsaturation.

KW - Catalytic hydrogenation

KW - Electron transport

KW - Lipid saturation

KW - Photosystem I

KW - Thermal stability

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JF - Biochimica et Biophysica Acta - Biomembranes

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