SAT: A late NS protein of porcine Parvovirus

Zoltán Zádori, József Szelei, Peter Tijssen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The genomes of all members of the Parvovirus genus were found to contain a small open reading frame (ORF), designated SAT, with a start codon four or seven nucleotides downstream of the VP2 initiation codon. Green fluorescent protein or FLAG fusion constructs of SAT demonstrated that these ORFs were expressed. Although the SAT proteins of the different parvoviruses are not particularly conserved, they were all predicted to contain a membrane-spanning helix, and mutations in this hydrophobic stretch affected the localization of the SAT protein. SAT colocalized with calreticulin in the membranes of the endoplasmic reticulum and the nucleus. A knockout mutant (SAT-), with an unmodified VP sequence, showed a "slow-spreading" phenotype. These knockout mutants could be complemented with VP2- SAT+ mutant. The SAT protein is a late non-structural (NS) protein, in contrast to previously identified NS proteins, since it is expressed from the same mRNA as VP2.

Original languageEnglish
Pages (from-to)13129-13138
Number of pages10
JournalJournal of Virology
Volume79
Issue number20
DOIs
Publication statusPublished - Oct 1 2005

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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