Sarcolipin, the "proteolipid" of skeletal muscle sarcoplasmic reticulum, is a unique, amphipathic, 31-residue peptide

Alicja Wawrzynow, Janet L. Theibert, Constance Murphy, I. Jóna, Anthony Martonosi, John H. Collins

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The sarcoplasmic reticulum of rabbit skeletal muscle contains a small "proteolipid," i.e., a protein which is soluble in acidic CHCl3 CH3OH. We propose the name sarcolipin for this small protein, to signify its lipid-like solubility and association with the sarcoplasmic reticulum. We have determined the following amino acid sequence for sarcolipin, using protein chemistry methods: M E R S T R E L C L N F T V V L I T V I L I W L L V R S Y Q Y. This 31-residue sequence includes a 19-residue hydrophobic segment which probably spans the sarcoplasmic reticulum membrane. The molecular weight calculated from the sequence, 3733, agrees with that measured by fast atom bombardment mass spectrometry, showing that sarcolipin contains no attached fatty acyl or other prosthetic groups.

Original languageEnglish
Pages (from-to)620-623
Number of pages4
JournalArchives of Biochemistry and Biophysics
Volume298
Issue number2
DOIs
Publication statusPublished - Nov 1 1992

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Proteolipids
Sarcoplasmic Reticulum
Muscle
Skeletal Muscle
Peptides
Fast Atom Bombardment Mass Spectrometry
Proteins
Prosthetics
Solubility
Names
Mass spectrometry
Amino Acid Sequence
Molecular Weight
Molecular weight
Association reactions
Rabbits
Membranes
Lipids
Amino Acids
Atoms

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sarcolipin, the "proteolipid" of skeletal muscle sarcoplasmic reticulum, is a unique, amphipathic, 31-residue peptide. / Wawrzynow, Alicja; Theibert, Janet L.; Murphy, Constance; Jóna, I.; Martonosi, Anthony; Collins, John H.

In: Archives of Biochemistry and Biophysics, Vol. 298, No. 2, 01.11.1992, p. 620-623.

Research output: Contribution to journalArticle

Wawrzynow, Alicja ; Theibert, Janet L. ; Murphy, Constance ; Jóna, I. ; Martonosi, Anthony ; Collins, John H. / Sarcolipin, the "proteolipid" of skeletal muscle sarcoplasmic reticulum, is a unique, amphipathic, 31-residue peptide. In: Archives of Biochemistry and Biophysics. 1992 ; Vol. 298, No. 2. pp. 620-623.
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