Sample preparation for the analysis of complex carbohydrates by multicapillary gel electrophoresis with light-emitting diode induced fluorescence detection

Marcell Olajos, Péter Hajós, Guenther K. Bonn, András Guttman

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

This paper evaluates various sample preparation methods for multicapillary gel electrophoresis based glycan analysis to support electrokinetic injection. First the removal of excess derivatization reagent is discussed. Although the Sephadex G10 filled multiscreen 96-well filter plate and Sephadex G10 filled pipet tips enabled increased analysis sensitivity, polyamide DPA-6S pipet tips worked particularly well. In this latter case an automated liquid handling system was used to increase purification throughput, necessary to feed the multicapillary electrophoresis unit. Problems associated with the high glucose content of such biological samples as normal human plasma were solved by applying ultrafiltration. Finally, a volatile buffer system was developed for exoglycosidase-based carbohydrate analysis.

Original languageEnglish
Pages (from-to)4241-4246
Number of pages6
JournalAnalytical Chemistry
Volume80
Issue number11
DOIs
Publication statusPublished - Jun 1 2008

ASJC Scopus subject areas

  • Analytical Chemistry

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