Sample handling in surface sensitive chemical and biological sensing: A practical review of basic fluidics and analyte transport

Norbert Orgovan, Daniel Patko, Csaba Hos, Sándor Kurunczi, B. Szabó, Jeremy J. Ramsden, R. Horváth

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This paper gives an overview of the advantages and associated caveats of the most common sample handling methods in surface-sensitive chemical and biological sensing. We summarize the basic theoretical and practical considerations one faces when designing and assembling the fluidic part of the sensor devices. The influence of analyte size, the use of closed and flow-through cuvettes, the importance of flow rate, tubing length and diameter, bubble traps, pressure-driven pumping, cuvette dead volumes, and sample injection systems are all discussed. Typical application areas of particular arrangements are also highlighted, such as the monitoring of cellular adhesion, biomolecule adsorption-desorption and ligand-receptor affinity binding. Our work is a practical review in the sense that for every sample handling arrangement considered we present our own experimental data and critically review our experience with the given arrangement. In the experimental part we focus on sample handling in optical waveguide lightmode spectroscopy (OWLS) measurements, but the present study is equally applicable for other biosensing technologies in which an analyte in solution is captured at a surface and its presence is monitored. Explicit attention is given to features that are expected to play an increasingly decisive role in determining the reliability of (bio)chemical sensing measurements, such as analyte transport to the sensor surface; the distorting influence of dead volumes in the fluidic system; and the appropriate sample handling of cell suspensions (e.g. their quasi-simultaneous deposition). At the appropriate places, biological aspects closely related to fluidics (e.g. cellular mechanotransduction, competitive adsorption, blood flow in veins) are also discussed, particularly with regard to their models used in biosensing.

Original languageEnglish
Pages (from-to)1-16
Number of pages16
JournalAdvances in Colloid and Interface Science
Volume211
DOIs
Publication statusPublished - 2014

Fingerprint

fluidics
Fluidics
Adsorption
Cellular Mechanotransduction
Veins
Spectrum Analysis
Suspensions
Sensors
Biomolecules
Optical waveguides
Tubing
Ligands
Technology
Pressure
Equipment and Supplies
Injections
Desorption
Blood
Adhesion
Flow rate

Keywords

  • Analyte transport
  • Dead volumes
  • Fluid handling of live cells
  • Fluidic systems
  • Label-free detection
  • Optical biosensors

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces
  • Medicine(all)

Cite this

Sample handling in surface sensitive chemical and biological sensing : A practical review of basic fluidics and analyte transport. / Orgovan, Norbert; Patko, Daniel; Hos, Csaba; Kurunczi, Sándor; Szabó, B.; Ramsden, Jeremy J.; Horváth, R.

In: Advances in Colloid and Interface Science, Vol. 211, 2014, p. 1-16.

Research output: Contribution to journalArticle

@article{ae64b2cc22d64566aa4947be86a6caf7,
title = "Sample handling in surface sensitive chemical and biological sensing: A practical review of basic fluidics and analyte transport",
abstract = "This paper gives an overview of the advantages and associated caveats of the most common sample handling methods in surface-sensitive chemical and biological sensing. We summarize the basic theoretical and practical considerations one faces when designing and assembling the fluidic part of the sensor devices. The influence of analyte size, the use of closed and flow-through cuvettes, the importance of flow rate, tubing length and diameter, bubble traps, pressure-driven pumping, cuvette dead volumes, and sample injection systems are all discussed. Typical application areas of particular arrangements are also highlighted, such as the monitoring of cellular adhesion, biomolecule adsorption-desorption and ligand-receptor affinity binding. Our work is a practical review in the sense that for every sample handling arrangement considered we present our own experimental data and critically review our experience with the given arrangement. In the experimental part we focus on sample handling in optical waveguide lightmode spectroscopy (OWLS) measurements, but the present study is equally applicable for other biosensing technologies in which an analyte in solution is captured at a surface and its presence is monitored. Explicit attention is given to features that are expected to play an increasingly decisive role in determining the reliability of (bio)chemical sensing measurements, such as analyte transport to the sensor surface; the distorting influence of dead volumes in the fluidic system; and the appropriate sample handling of cell suspensions (e.g. their quasi-simultaneous deposition). At the appropriate places, biological aspects closely related to fluidics (e.g. cellular mechanotransduction, competitive adsorption, blood flow in veins) are also discussed, particularly with regard to their models used in biosensing.",
keywords = "Analyte transport, Dead volumes, Fluid handling of live cells, Fluidic systems, Label-free detection, Optical biosensors",
author = "Norbert Orgovan and Daniel Patko and Csaba Hos and S{\'a}ndor Kurunczi and B. Szab{\'o} and Ramsden, {Jeremy J.} and R. Horv{\'a}th",
year = "2014",
doi = "10.1016/j.cis.2014.03.011",
language = "English",
volume = "211",
pages = "1--16",
journal = "Advances in Colloid and Interface Science",
issn = "0001-8686",
publisher = "Elsevier",

}

TY - JOUR

T1 - Sample handling in surface sensitive chemical and biological sensing

T2 - A practical review of basic fluidics and analyte transport

AU - Orgovan, Norbert

AU - Patko, Daniel

AU - Hos, Csaba

AU - Kurunczi, Sándor

AU - Szabó, B.

AU - Ramsden, Jeremy J.

AU - Horváth, R.

PY - 2014

Y1 - 2014

N2 - This paper gives an overview of the advantages and associated caveats of the most common sample handling methods in surface-sensitive chemical and biological sensing. We summarize the basic theoretical and practical considerations one faces when designing and assembling the fluidic part of the sensor devices. The influence of analyte size, the use of closed and flow-through cuvettes, the importance of flow rate, tubing length and diameter, bubble traps, pressure-driven pumping, cuvette dead volumes, and sample injection systems are all discussed. Typical application areas of particular arrangements are also highlighted, such as the monitoring of cellular adhesion, biomolecule adsorption-desorption and ligand-receptor affinity binding. Our work is a practical review in the sense that for every sample handling arrangement considered we present our own experimental data and critically review our experience with the given arrangement. In the experimental part we focus on sample handling in optical waveguide lightmode spectroscopy (OWLS) measurements, but the present study is equally applicable for other biosensing technologies in which an analyte in solution is captured at a surface and its presence is monitored. Explicit attention is given to features that are expected to play an increasingly decisive role in determining the reliability of (bio)chemical sensing measurements, such as analyte transport to the sensor surface; the distorting influence of dead volumes in the fluidic system; and the appropriate sample handling of cell suspensions (e.g. their quasi-simultaneous deposition). At the appropriate places, biological aspects closely related to fluidics (e.g. cellular mechanotransduction, competitive adsorption, blood flow in veins) are also discussed, particularly with regard to their models used in biosensing.

AB - This paper gives an overview of the advantages and associated caveats of the most common sample handling methods in surface-sensitive chemical and biological sensing. We summarize the basic theoretical and practical considerations one faces when designing and assembling the fluidic part of the sensor devices. The influence of analyte size, the use of closed and flow-through cuvettes, the importance of flow rate, tubing length and diameter, bubble traps, pressure-driven pumping, cuvette dead volumes, and sample injection systems are all discussed. Typical application areas of particular arrangements are also highlighted, such as the monitoring of cellular adhesion, biomolecule adsorption-desorption and ligand-receptor affinity binding. Our work is a practical review in the sense that for every sample handling arrangement considered we present our own experimental data and critically review our experience with the given arrangement. In the experimental part we focus on sample handling in optical waveguide lightmode spectroscopy (OWLS) measurements, but the present study is equally applicable for other biosensing technologies in which an analyte in solution is captured at a surface and its presence is monitored. Explicit attention is given to features that are expected to play an increasingly decisive role in determining the reliability of (bio)chemical sensing measurements, such as analyte transport to the sensor surface; the distorting influence of dead volumes in the fluidic system; and the appropriate sample handling of cell suspensions (e.g. their quasi-simultaneous deposition). At the appropriate places, biological aspects closely related to fluidics (e.g. cellular mechanotransduction, competitive adsorption, blood flow in veins) are also discussed, particularly with regard to their models used in biosensing.

KW - Analyte transport

KW - Dead volumes

KW - Fluid handling of live cells

KW - Fluidic systems

KW - Label-free detection

KW - Optical biosensors

UR - http://www.scopus.com/inward/record.url?scp=84905408456&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905408456&partnerID=8YFLogxK

U2 - 10.1016/j.cis.2014.03.011

DO - 10.1016/j.cis.2014.03.011

M3 - Article

C2 - 24846752

AN - SCOPUS:84905408456

VL - 211

SP - 1

EP - 16

JO - Advances in Colloid and Interface Science

JF - Advances in Colloid and Interface Science

SN - 0001-8686

ER -