Sample and probe: A novel approach for identifying development-specific cis-elements of the enkephalin gene

Albert Dobi, M. Palkóvits, Mary A. Ring, Alex Eitel, Christina G. Palkovits, Francis Lim, Denes V. Agoston

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have developed a novel 'sample and probe' approach as a means to identifying specific DNA elements of the enkephalin gene that control differentiation of the enkephalinergic phenotype during neurodevelopment. The approach is a systematic spatiotemporal analysis of protein-DNA interactions; soluble nuclear proteins ('samples') prepared from microdissected regions of the developing brain are 'probed' with radiolabeled DNA fragments representing various regulatory regions of the enkephalin gene. The resulting spatiotemporal 'molecular maps', i.e. characteristic patterns of protein-DNA complexes showed DNA regions that harbor potential cis-elements regulating differentiation of the enkephalin phenotype at various stages of neurodevelopment. DNase I footprint analysis of such a DNA region identified a binding site (GACGGGAGATCGCTCGT) which is similar to the motif for a lymphoid-specific, developmentally regulated transcription factor, Ikaros, suggesting that the developing brain expresses Ikaros-like transcription factor(s) in a spatiotemporally defined manner. In summary, our approach offers a unique view into the chronology of coordinated protein-DNA interactions and will greatly facilitate identifying DNA elements and isolating development-specific transcription factors.

Original languageEnglish
Pages (from-to)98-111
Number of pages14
JournalMolecular Brain Research
Volume52
Issue number1
DOIs
Publication statusPublished - Dec 1 1997

Fingerprint

Enkephalins
DNA
Genes
Ikaros Transcription Factor
Spatio-Temporal Analysis
Phenotype
Chronology
Proteins
Nucleic Acid Regulatory Sequences
Deoxyribonuclease I
Brain
Nuclear Proteins
Transcription Factors
Binding Sites

Keywords

  • Development
  • Enkephalin gene
  • Microdissection
  • Protein-DNA interaction
  • Regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Sample and probe : A novel approach for identifying development-specific cis-elements of the enkephalin gene. / Dobi, Albert; Palkóvits, M.; Ring, Mary A.; Eitel, Alex; Palkovits, Christina G.; Lim, Francis; Agoston, Denes V.

In: Molecular Brain Research, Vol. 52, No. 1, 01.12.1997, p. 98-111.

Research output: Contribution to journalArticle

Dobi, Albert ; Palkóvits, M. ; Ring, Mary A. ; Eitel, Alex ; Palkovits, Christina G. ; Lim, Francis ; Agoston, Denes V. / Sample and probe : A novel approach for identifying development-specific cis-elements of the enkephalin gene. In: Molecular Brain Research. 1997 ; Vol. 52, No. 1. pp. 98-111.
@article{7593873e496542099aa0fe0b09f8855d,
title = "Sample and probe: A novel approach for identifying development-specific cis-elements of the enkephalin gene",
abstract = "We have developed a novel 'sample and probe' approach as a means to identifying specific DNA elements of the enkephalin gene that control differentiation of the enkephalinergic phenotype during neurodevelopment. The approach is a systematic spatiotemporal analysis of protein-DNA interactions; soluble nuclear proteins ('samples') prepared from microdissected regions of the developing brain are 'probed' with radiolabeled DNA fragments representing various regulatory regions of the enkephalin gene. The resulting spatiotemporal 'molecular maps', i.e. characteristic patterns of protein-DNA complexes showed DNA regions that harbor potential cis-elements regulating differentiation of the enkephalin phenotype at various stages of neurodevelopment. DNase I footprint analysis of such a DNA region identified a binding site (GACGGGAGATCGCTCGT) which is similar to the motif for a lymphoid-specific, developmentally regulated transcription factor, Ikaros, suggesting that the developing brain expresses Ikaros-like transcription factor(s) in a spatiotemporally defined manner. In summary, our approach offers a unique view into the chronology of coordinated protein-DNA interactions and will greatly facilitate identifying DNA elements and isolating development-specific transcription factors.",
keywords = "Development, Enkephalin gene, Microdissection, Protein-DNA interaction, Regulation",
author = "Albert Dobi and M. Palk{\'o}vits and Ring, {Mary A.} and Alex Eitel and Palkovits, {Christina G.} and Francis Lim and Agoston, {Denes V.}",
year = "1997",
month = "12",
day = "1",
doi = "10.1016/S0169-328X(97)00238-6",
language = "English",
volume = "52",
pages = "98--111",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Sample and probe

T2 - A novel approach for identifying development-specific cis-elements of the enkephalin gene

AU - Dobi, Albert

AU - Palkóvits, M.

AU - Ring, Mary A.

AU - Eitel, Alex

AU - Palkovits, Christina G.

AU - Lim, Francis

AU - Agoston, Denes V.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - We have developed a novel 'sample and probe' approach as a means to identifying specific DNA elements of the enkephalin gene that control differentiation of the enkephalinergic phenotype during neurodevelopment. The approach is a systematic spatiotemporal analysis of protein-DNA interactions; soluble nuclear proteins ('samples') prepared from microdissected regions of the developing brain are 'probed' with radiolabeled DNA fragments representing various regulatory regions of the enkephalin gene. The resulting spatiotemporal 'molecular maps', i.e. characteristic patterns of protein-DNA complexes showed DNA regions that harbor potential cis-elements regulating differentiation of the enkephalin phenotype at various stages of neurodevelopment. DNase I footprint analysis of such a DNA region identified a binding site (GACGGGAGATCGCTCGT) which is similar to the motif for a lymphoid-specific, developmentally regulated transcription factor, Ikaros, suggesting that the developing brain expresses Ikaros-like transcription factor(s) in a spatiotemporally defined manner. In summary, our approach offers a unique view into the chronology of coordinated protein-DNA interactions and will greatly facilitate identifying DNA elements and isolating development-specific transcription factors.

AB - We have developed a novel 'sample and probe' approach as a means to identifying specific DNA elements of the enkephalin gene that control differentiation of the enkephalinergic phenotype during neurodevelopment. The approach is a systematic spatiotemporal analysis of protein-DNA interactions; soluble nuclear proteins ('samples') prepared from microdissected regions of the developing brain are 'probed' with radiolabeled DNA fragments representing various regulatory regions of the enkephalin gene. The resulting spatiotemporal 'molecular maps', i.e. characteristic patterns of protein-DNA complexes showed DNA regions that harbor potential cis-elements regulating differentiation of the enkephalin phenotype at various stages of neurodevelopment. DNase I footprint analysis of such a DNA region identified a binding site (GACGGGAGATCGCTCGT) which is similar to the motif for a lymphoid-specific, developmentally regulated transcription factor, Ikaros, suggesting that the developing brain expresses Ikaros-like transcription factor(s) in a spatiotemporally defined manner. In summary, our approach offers a unique view into the chronology of coordinated protein-DNA interactions and will greatly facilitate identifying DNA elements and isolating development-specific transcription factors.

KW - Development

KW - Enkephalin gene

KW - Microdissection

KW - Protein-DNA interaction

KW - Regulation

UR - http://www.scopus.com/inward/record.url?scp=0030775153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030775153&partnerID=8YFLogxK

U2 - 10.1016/S0169-328X(97)00238-6

DO - 10.1016/S0169-328X(97)00238-6

M3 - Article

C2 - 9450682

AN - SCOPUS:0030775153

VL - 52

SP - 98

EP - 111

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -