Salt effects on the bacteriophage T7-II structure and activity changes.

K. Tóth, G. Csík, G. Rontó

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Optically detected thermal stability and biological activity of phage T7 has been compared as the function of the ionic composition and strength of the buffers. The ionic strength range was studied between 20-140 mmol/1. In Tris buffer containing only monovalent ions the biological activity of the phages decreases abruptly below 50 mmol/1 ionic strength. Structural studies show a logarithmic dependence between the ionic strength and the intraphage DNA stability and no significant change in the thermal stability of the whole phage. Mg2+ and Ca2+ ions at low concentration (1 mmol/1) given into a Tris buffer of 20 mmol/1 original ionic strength highly stabilize the biological activity, which stabilization is also to be seen in the intraphage DNA and also in the whole phage thermal denaturation process.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalPhysiological Chemistry and Physics and Medical NMR
Volume19
Issue number1
Publication statusPublished - 1987

Fingerprint

Bacteriophage T7
Bacteriophages
Ionic strength
Osmolar Concentration
Salts
Bioactivity
Tromethamine
Hot Temperature
Thermodynamic stability
Ions
Denaturation
DNA
Buffers
Stabilization
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Physiology
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Salt effects on the bacteriophage T7-II structure and activity changes. / Tóth, K.; Csík, G.; Rontó, G.

In: Physiological Chemistry and Physics and Medical NMR, Vol. 19, No. 1, 1987, p. 67-74.

Research output: Contribution to journalArticle

@article{d39a8f97fc114465a1bf21a4afb09f48,
title = "Salt effects on the bacteriophage T7-II structure and activity changes.",
abstract = "Optically detected thermal stability and biological activity of phage T7 has been compared as the function of the ionic composition and strength of the buffers. The ionic strength range was studied between 20-140 mmol/1. In Tris buffer containing only monovalent ions the biological activity of the phages decreases abruptly below 50 mmol/1 ionic strength. Structural studies show a logarithmic dependence between the ionic strength and the intraphage DNA stability and no significant change in the thermal stability of the whole phage. Mg2+ and Ca2+ ions at low concentration (1 mmol/1) given into a Tris buffer of 20 mmol/1 original ionic strength highly stabilize the biological activity, which stabilization is also to be seen in the intraphage DNA and also in the whole phage thermal denaturation process.",
author = "K. T{\'o}th and G. Cs{\'i}k and G. Ront{\'o}",
year = "1987",
language = "English",
volume = "19",
pages = "67--74",
journal = "Physiological chemistry and physics",
issn = "0748-6642",
publisher = "Pacific Press Inc.",
number = "1",

}

TY - JOUR

T1 - Salt effects on the bacteriophage T7-II structure and activity changes.

AU - Tóth, K.

AU - Csík, G.

AU - Rontó, G.

PY - 1987

Y1 - 1987

N2 - Optically detected thermal stability and biological activity of phage T7 has been compared as the function of the ionic composition and strength of the buffers. The ionic strength range was studied between 20-140 mmol/1. In Tris buffer containing only monovalent ions the biological activity of the phages decreases abruptly below 50 mmol/1 ionic strength. Structural studies show a logarithmic dependence between the ionic strength and the intraphage DNA stability and no significant change in the thermal stability of the whole phage. Mg2+ and Ca2+ ions at low concentration (1 mmol/1) given into a Tris buffer of 20 mmol/1 original ionic strength highly stabilize the biological activity, which stabilization is also to be seen in the intraphage DNA and also in the whole phage thermal denaturation process.

AB - Optically detected thermal stability and biological activity of phage T7 has been compared as the function of the ionic composition and strength of the buffers. The ionic strength range was studied between 20-140 mmol/1. In Tris buffer containing only monovalent ions the biological activity of the phages decreases abruptly below 50 mmol/1 ionic strength. Structural studies show a logarithmic dependence between the ionic strength and the intraphage DNA stability and no significant change in the thermal stability of the whole phage. Mg2+ and Ca2+ ions at low concentration (1 mmol/1) given into a Tris buffer of 20 mmol/1 original ionic strength highly stabilize the biological activity, which stabilization is also to be seen in the intraphage DNA and also in the whole phage thermal denaturation process.

UR - http://www.scopus.com/inward/record.url?scp=0023082395&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023082395&partnerID=8YFLogxK

M3 - Article

C2 - 3303076

AN - SCOPUS:0023082395

VL - 19

SP - 67

EP - 74

JO - Physiological chemistry and physics

JF - Physiological chemistry and physics

SN - 0748-6642

IS - 1

ER -