S-genotyping of old apple cultivars from the Carpathian basin: Methodological, breeding and evolutionary aspects

Julia Halász, A. Hegedüs, Zsuzsanna György, E. Pállinger, Magdolna Tóth

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Apple exhibits self-incompatibility controlled by the multiallelic S-locus. Twenty-three old apple cultivars were S-genotyped using three different approaches (allele-specific polymerase chain reaction (PCR) + cleaved amplified polymorphic sequences (CAPS), consensus PCR + sequencing and consensus PCR + CAPS) to compare the robustness and reliability of these techniques and characterise genotypes from the Carpathian basin that might be useful in resistance breeding. Best results were obtained using the ASPF3 and ASPR3S consensus primer pair that detected 96% of all alleles carried by the 23 cultivars tested. Flow cytometry analysis was also needed to control the completeness of the genotypes as was seen in case of a tetraploid cultivar with only three assigned S-alleles. The genetic disparity between the old Carpathian basin and modern apple cultivars was indicated by differences in allele frequency data (S9, S24 and S26) as well as single nucleotide polymorphisms in S1, S2, S7S24 and S26 and indels in S20 and S26 alleles. An alignment of partial genomic sequences indicated trans-specific and trans-generic evolution of S-ribonuclease alleles in the Maloideae subfamily (S26 and S28) and a possibly recent introgression event (S1) between Malus × domestica and Malus sylvestris. These data suggest that the genome of old cultivars from the Carpathian basin was enriched by several Malus taxa and are free from the consequences of modern breeding. These cultivars may contribute to the widening of the genetic basis of cultivated apple and prevent genetic erosion in future commercial cultivars.

Original languageEnglish
Pages (from-to)1135-1145
Number of pages11
JournalTree Genetics and Genomes
Volume7
Issue number6
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Malus
genotyping
Breeding
cultivar
apples
breeding
allele
basins
cultivars
Alleles
basin
alleles
Malus domestica
polymerase chain reaction
Polymerase Chain Reaction
genotype
Genotype
self incompatibility
Tetraploidy
ribonucleases

Keywords

  • Apple
  • Ploidy
  • S-allele diversity
  • S-genotyping
  • S-RNase
  • Self-incompatibility
  • SNP

ASJC Scopus subject areas

  • Forestry
  • Horticulture
  • Genetics
  • Molecular Biology

Cite this

S-genotyping of old apple cultivars from the Carpathian basin : Methodological, breeding and evolutionary aspects. / Halász, Julia; Hegedüs, A.; György, Zsuzsanna; Pállinger, E.; Tóth, Magdolna.

In: Tree Genetics and Genomes, Vol. 7, No. 6, 12.2011, p. 1135-1145.

Research output: Contribution to journalArticle

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