S-genotyping of Eastern European almond cultivars: identification and characterization of new (S36-S39) self-incompatibility ribonuclease alleles

J. Halász, Á Fodor, A. Pedryc, A. Hegedus

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

With 3 figures and 2 tablesConsensus polymerase chain reaction (PCR) primers amplifying the first and second intron regions of the S-RNase gene and DNA sequencing as well as fruit set analysis after controlled pollinations were used for S-genotyping of Eastern European almond cultivars. Complete S-genotypes have been proposed for 19 accessions, and partial S-genotypes have been proposed for three additional accessions. Based on DNA sequences and fruit set analysis, two novel cross-incompatibility groups have been proposed: group XXI (S11S31H) including 'Szigetcsépi 55' (3/9) and 'Eriane' and group XXII (S36S37) for 'Nikitskyi' and 'Óriás kagyló' (4/4). Four new alleles were identified, S36 (FJ529211), S37 (FJ529212), S38 (FJ529213) and S39 (FJ529214). The combination of consensus PCR primers amplifying the first and second intron regions could reliably discriminate the S-RNase alleles identified in this study from those described previously. Our results supply long-awaited information on almond S-allele diversity from regions between the main cultivation centres and the centre of origin of this species.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalPlant Breeding
Volume129
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

Keywords

  • Breeding
  • Prunus dulcis
  • S-RNase
  • S-allele diversity
  • S-genotyping
  • Self-incompatibility

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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