S-allele constitution of hexaploid European plum cultivars

N. Makovics-Zsohár, A. Hegedüs, J. Halász

Research output: Contribution to journalArticle

Abstract

European plum (Prunus domestica) is an important fruit crop in Central Europe with a complex hexaploid genome of unknown origin. The characterization of the Slocus of 17 economically important European plum cultivars was carried out. To characterize the length polymorphism for first and second introns, the PaConsI and EM-PC primer pairs were used. Altogether, 18 different alleles were identified, of which 11 S-RNase alleles were first detected. To confirm S-allele identity, partial S-RNase sequences were determined and blasted on the GenBank database. Partial S-haplotype-specific F-box alleles were also cloned and sequenced. Several trans-specific alleles were detected and their phylogenetic analysis and Tajima’s D test showed the rate of evolution dramatically accelerates when allele specificity is modified by fixed amino acid replacements. A total of 17 different S-genotypes were assigned, so the discrimination of all assayed plum cultivars was successful based on their unique Sgenotypes. Cross-incompatible cultivars were not found. Our analysis is continued to identify mutations in the S-locus genes that might be responsible for the transition from a self-incompatible to a self-compatible phenotype in a hexaploid species.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalActa Horticulturae
Volume1231
DOIs
Publication statusPublished - Jan 1 2019

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Keywords

  • Evolution
  • Intron length polymorphism
  • Prunus domestica
  • S-genotype
  • S-RNase

ASJC Scopus subject areas

  • Horticulture

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