S-allele constitution of hexaploid European plum cultivars

N. Makovics-Zsohár, A. Hegedüs, J. Halász

Research output: Contribution to journalArticle

Abstract

European plum (Prunus domestica) is an important fruit crop in Central Europe with a complex hexaploid genome of unknown origin. The characterization of the Slocus of 17 economically important European plum cultivars was carried out. To characterize the length polymorphism for first and second introns, the PaConsI and EM-PC primer pairs were used. Altogether, 18 different alleles were identified, of which 11 S-RNase alleles were first detected. To confirm S-allele identity, partial S-RNase sequences were determined and blasted on the GenBank database. Partial S-haplotype-specific F-box alleles were also cloned and sequenced. Several trans-specific alleles were detected and their phylogenetic analysis and Tajima’s D test showed the rate of evolution dramatically accelerates when allele specificity is modified by fixed amino acid replacements. A total of 17 different S-genotypes were assigned, so the discrimination of all assayed plum cultivars was successful based on their unique Sgenotypes. Cross-incompatible cultivars were not found. Our analysis is continued to identify mutations in the S-locus genes that might be responsible for the transition from a self-incompatible to a self-compatible phenotype in a hexaploid species.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalActa Horticulturae
Volume1231
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Prunus domestica
hexaploidy
alleles
cultivars
fruit crops
plums
Central European region
introns
haplotypes
genetic polymorphism
mutation
phenotype
loci
amino acids
genome
genotype
phylogeny
genes

Keywords

  • Evolution
  • Intron length polymorphism
  • Prunus domestica
  • S-genotype
  • S-RNase

ASJC Scopus subject areas

  • Horticulture

Cite this

S-allele constitution of hexaploid European plum cultivars. / Makovics-Zsohár, N.; Hegedüs, A.; Halász, J.

In: Acta Horticulturae, Vol. 1231, 01.01.2019, p. 151-155.

Research output: Contribution to journalArticle

Makovics-Zsohár, N. ; Hegedüs, A. ; Halász, J. / S-allele constitution of hexaploid European plum cultivars. In: Acta Horticulturae. 2019 ; Vol. 1231. pp. 151-155.
@article{d4bdbea254494bf4a7eea3f564997e8f,
title = "S-allele constitution of hexaploid European plum cultivars",
abstract = "European plum (Prunus domestica) is an important fruit crop in Central Europe with a complex hexaploid genome of unknown origin. The characterization of the Slocus of 17 economically important European plum cultivars was carried out. To characterize the length polymorphism for first and second introns, the PaConsI and EM-PC primer pairs were used. Altogether, 18 different alleles were identified, of which 11 S-RNase alleles were first detected. To confirm S-allele identity, partial S-RNase sequences were determined and blasted on the GenBank database. Partial S-haplotype-specific F-box alleles were also cloned and sequenced. Several trans-specific alleles were detected and their phylogenetic analysis and Tajima’s D test showed the rate of evolution dramatically accelerates when allele specificity is modified by fixed amino acid replacements. A total of 17 different S-genotypes were assigned, so the discrimination of all assayed plum cultivars was successful based on their unique Sgenotypes. Cross-incompatible cultivars were not found. Our analysis is continued to identify mutations in the S-locus genes that might be responsible for the transition from a self-incompatible to a self-compatible phenotype in a hexaploid species.",
keywords = "Evolution, Intron length polymorphism, Prunus domestica, S-genotype, S-RNase",
author = "N. Makovics-Zsoh{\'a}r and A. Heged{\"u}s and J. Hal{\'a}sz",
year = "2019",
month = "1",
day = "1",
doi = "10.17660/ActaHortic.2019.1231.25",
language = "English",
volume = "1231",
pages = "151--155",
journal = "Acta Horticulturae",
issn = "0567-7572",
publisher = "International Society for Horticultural Science",

}

TY - JOUR

T1 - S-allele constitution of hexaploid European plum cultivars

AU - Makovics-Zsohár, N.

AU - Hegedüs, A.

AU - Halász, J.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - European plum (Prunus domestica) is an important fruit crop in Central Europe with a complex hexaploid genome of unknown origin. The characterization of the Slocus of 17 economically important European plum cultivars was carried out. To characterize the length polymorphism for first and second introns, the PaConsI and EM-PC primer pairs were used. Altogether, 18 different alleles were identified, of which 11 S-RNase alleles were first detected. To confirm S-allele identity, partial S-RNase sequences were determined and blasted on the GenBank database. Partial S-haplotype-specific F-box alleles were also cloned and sequenced. Several trans-specific alleles were detected and their phylogenetic analysis and Tajima’s D test showed the rate of evolution dramatically accelerates when allele specificity is modified by fixed amino acid replacements. A total of 17 different S-genotypes were assigned, so the discrimination of all assayed plum cultivars was successful based on their unique Sgenotypes. Cross-incompatible cultivars were not found. Our analysis is continued to identify mutations in the S-locus genes that might be responsible for the transition from a self-incompatible to a self-compatible phenotype in a hexaploid species.

AB - European plum (Prunus domestica) is an important fruit crop in Central Europe with a complex hexaploid genome of unknown origin. The characterization of the Slocus of 17 economically important European plum cultivars was carried out. To characterize the length polymorphism for first and second introns, the PaConsI and EM-PC primer pairs were used. Altogether, 18 different alleles were identified, of which 11 S-RNase alleles were first detected. To confirm S-allele identity, partial S-RNase sequences were determined and blasted on the GenBank database. Partial S-haplotype-specific F-box alleles were also cloned and sequenced. Several trans-specific alleles were detected and their phylogenetic analysis and Tajima’s D test showed the rate of evolution dramatically accelerates when allele specificity is modified by fixed amino acid replacements. A total of 17 different S-genotypes were assigned, so the discrimination of all assayed plum cultivars was successful based on their unique Sgenotypes. Cross-incompatible cultivars were not found. Our analysis is continued to identify mutations in the S-locus genes that might be responsible for the transition from a self-incompatible to a self-compatible phenotype in a hexaploid species.

KW - Evolution

KW - Intron length polymorphism

KW - Prunus domestica

KW - S-genotype

KW - S-RNase

UR - http://www.scopus.com/inward/record.url?scp=85061299323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061299323&partnerID=8YFLogxK

U2 - 10.17660/ActaHortic.2019.1231.25

DO - 10.17660/ActaHortic.2019.1231.25

M3 - Article

VL - 1231

SP - 151

EP - 155

JO - Acta Horticulturae

JF - Acta Horticulturae

SN - 0567-7572

ER -